Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Voshol, PJ; Jong, MC; Dahlmans, VE; Kratky, D; Levak-Frank, S; Zechner, R; Romijn, JA; Havekes, LM.
In muscle-specific lipoprotein lipase-overexpressing mice, muscle triglyceride content is increased without inhibition of insulin-stimulated whole-body and muscle-specific glucose uptake.
Diabetes. 2001; 50(11):2585-2590
Doi: 10.2337/diabetes.50.11.2585
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- Co-Autor*innen der Med Uni Graz
- Kratky Dagmar
- Levak Sanja
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- Abstract:
- In patients with type 2 diabetes, a strong correlation between accumulation of intramuscular triclycerides (TGs) and insulin resistance has been found. The aim of the present study was to determine whether there is a causal relation between intramuscular TG accumulation and insulin sensitivity. Therefore, in mice with muscle-specific overexpression of human lipoprotein lipase (LPL) and control mice, muscle TG content was measured in combination with glucose uptake in vivo, under hyperinsulinemic-euglycemic conditions. Overexpression of LPL in muscle resulted in accumulation of TGs in skeletal muscle (85.5 +/- 33.3 vs. 25.7 +/- 23.1 micromol/g tissue in LPL and control mice, respectively; P < 0.05). During the hyperinsulinemic clamp study, there were no differences in plasma glucose, insulin, and FFA concentrations between the two groups. Moreover, whole-body, as well as skeletal muscle, insulin-mediated glucose uptake did not differ between LPL-overexpressing and wild-type mice. Surprisingly, whole-body glucose oxidation was decreased by approximately 60% (P < 0.05), whereas nonoxidative glucose disposal was increased by approximately 50% (P < 0.05) in LPL-overexpressing versus control mice. In conclusion, overexpression of human LPL in muscle increases intramuscular TG accumulation, but does not affect whole-body or muscle-specific insulin-mediated uptake, findings that argue against a simple causal relation between intramuscular TG content and insulin resistance.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Blood - metabolism
- Deoxyglucose - pharmacokinetics
- Glucose - metabolism
- Glycogen - biosynthesis
- Humans - biosynthesis
- Insulin - physiology
- Lipoprotein Lipase - metabolism
- Male - metabolism
- Mice - metabolism
- Mice, Transgenic - metabolism
- Muscle, Skeletal - enzymology
- Reference Values - enzymology
- Triglycerides - metabolism