Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wagner, T; Schwartz, DW; Winter, M; Kabrna, E; Kollars, M; Schwarzinger, I; Lanzer, G; Mayr, WR; Geissler, K.
Kinetics of CFU-Mk after automated plateletpheresis.
Vox Sang. 2001; 81(3):167-171
Doi: 10.1046%2Fj.0042-9007.2001.00096.x
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Wagner Thomas
- Co-Autor*innen der Med Uni Graz
- Lanzer Gerhard
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- BACKGROUND AND OBJECTIVES: Platelet count, thrombopoetin (TPO) level and the compartment of megakaryocyte progenitor cells (CFU-Mk) are major determinants in the regulation of thrombopoiesis. The aim of this study was to investigate the potential changes in the compartment of CFU-Mk and their correlation with serum TPO levels and platelet count after plateletpheresis. MATERIALS AND METHODS: Twelve healthy individuals were randomly assigned to undergo single-donor plateletpheresis. A collagen-based in vitro culture system was used to determine the number of peripheral blood (PB) CFU-Mk before and after donation and on days 1, 4 and 7 thereafter. TPO levels were measured by a specific enzyme-linked immunosorbent assay and whole blood counts were performed using an automated cell counter. RESULTS: The pre-apheresis platelet count (mean +/- SEM: 276 +/- 13 x 10(9)/l) decreased after plateletpheresis to a nadir of 194 +/- 8 x 10(9)/l (P < 0.001), showed a gradual increase on days 1 and 4, and reached pre-apheresis values by day 7 (280 +/- 11). The serum TPO levels were found to be significantly increased on days 1 and 7 as compared to baseline levels (baseline value 103.3 +/- 18.5 pg/ml versus day-1 value 135.8 +/- 25.8 pg/ml and day-7 value 132 +/- 30.19 pg/ml; P < 0.03 and P < 0.03, respectively). The numbers of CFU-Mk in PB were significantly elevated on day 4 only (125 +/- 21 colonies/ml of PB versus pre-apheresis values of 68 +/- 18 colonies/ml of PB, P < 0.005). CONCLUSIONS: These findings suggest that platelet loss during plateletpheresis affects thrombopoiesis at the progenitor cell level, probably through alterations in TPO plasma concentrations.
- Find related publications in this database (using NLM MeSH Indexing)
- Blood Cell Count -
- Cell Culture Techniques - methods
- Hematopoiesis - methods
- Humans - methods
- Kinetics - methods
- Megakaryocytes - cytology
- Myeloid Progenitor Cells - cytology
- Platelet Count - instrumentation
- Plateletpheresis - instrumentation
- Thrombopoietin - blood
- Find related publications in this database (Keywords)
- CFU-Mk
- plateletpheresis
- platelets
- TPO