Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Nesbit, M; Schaider, H; Berking, C; Shih, DT; Hsu, MY; McBrian, M; Crombleholme, TM; Elenitsas, R; Buck, C; Herlyn, M.
Alpha5 and alpha2 integrin gene transfers mimic the PDGF-B-induced transformed phenotype of fibroblasts in human skin.
Lab Invest. 2001; 81(9):1263-1274
Doi: 10.1038/labinvest.3780340
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- Co-Autor*innen der Med Uni Graz
- Schaider Helmut
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- Abstract:
- Platelet-derived growth factor (PDGF)-B is a proto-oncogene capable of transforming fibroblasts. Using adenoviral vectors, we tested whether endogenous PDGF-B expression in human skin xenotransplants leads to changes in the expression of alpha5 and alpha2 integrin subunits and whether integrin overexpression leads to PDGF-related changes in the skin. In vitro, transduction of fibroblasts with PDGF-B or the integrin alpha5 subunit stimulated multilayered growth and spindle-type morphology, both markers of mesenchymal cell transformation. In vivo, PDGF-B transduction of the human dermis was associated with up-regulation of collagen and fibronectin synthesis, increases in alpha5 and alpha2 integrin subunit expression, vessel formation, and proliferation of fibroblasts, keratinocytes, and pericytes. A similar stromal response was induced when alpha5 and alpha2 integrin subunits were overexpressed in the human dermis, suggesting that integrins play a major role in the induction of a transformed phenotype of fibroblasts by PDGF-B.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD - genetics
- Cell Line - genetics
- Cell Survival - genetics
- Fibroblasts - drug effects
- Gene Transfer Techniques - drug effects
- Humans - drug effects
- Integrin alpha2 - drug effects
- Integrin alpha5 - drug effects
- Phenotype - drug effects
- Proto-Oncogene Proteins c-sis - genetics
- Skin - cytology
- Skin Physiology - cytology
- Transduction, Genetic - cytology