Medizinische Universität Graz - Research portal

Go directly to the nagivation.
Go directly to the content.

Navigation/Search

Selected Publication:

Kessler, HH; Deuretzbacher, D; Stelzl, E; Daghofer, E; Santner, BI; Marth, E.
Determination of human immunodeficiency virus type 1 subtypes by a rapid method useful for the routine diagnostic laboratory.
Clin Diagn Lab Immunol. 2001; 8(5):1018-1020 Doi: 10.1128/CDLI.8.5.1018-1020.2001 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

Leading authors Med Uni Graz: Kessler Harald
Co-authors Med Uni Graz: Daghofer Elisabeth; Marth Egon; Santner Brigitte; Stelzl Evelyn
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: The existence of human immunodeficiency virus type 1 (HIV-1) subtypes has many important implications for the global evolution of HIV and for the evaluation of pathogenicity, transmissibility, and candidate HIV vaccines. The aim of this study was to establish a rapid method for determination of HIV-1 subtypes useful for a routine diagnostic laboratory and to investigate the distribution of HIV-1 subtypes in Austrian patients. Samples were tested by a subtyping method based on a 1.3-kb sequence of the polymerase gene generated by a commercially available drug resistance assay. The generated sequence was subtyped by means of an HIV sequence database. Results of 74 routine samples revealed subtype B (71.6%) as the predominant subtype, followed by subtype A (13.5%) and subtype C (6.8%). Subtypes E, F, G, and AE (CM240) were also detected. This subtyping method was found to be very easy to handle, rapid, and inexpensive and has proved suitable for high-throughput routine diagnostic laboratories. The specific polymerase gene sequence, however, must be existent.
Find related publications in this database (using NLM MeSH Indexing): Child -; DNA Polymerase beta - genetics; Female -; HIV Infections - enzymology HIV Infections - virology; HIV Reverse Transcriptase - genetics; HIV-1 - classification HIV-1 - genetics; Humans -; Male -; RNA Polymerase II - genetics; Reagent Kits, Diagnostic - virology; Serotyping - methods