Medizinische Universität Graz - Research portal
Selected Publication:
Pouly, S; Storch, MK; Matthieu, JM; Lassmann, H; Monnet-Tschudi, F; Honegger, P.
Demyelination induced by protein kinase C-activating tumor promoters in aggregating brain cell cultures.
J Neurosci Res. 1997; 49(2):121-132
Doi: 10.1002/(SICI)1097-4547(19970715)49:2<121::AID-JNR1>3.0.CO;2-B
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- Co-authors Med Uni Graz
- Storch Maria
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- Abstract:
- The plasticity of mature oligodendrocytes was studied in aggregating brain cell cultures at the period of maximal expression of myelin marker proteins. The protein kinase C (PKC)-activating tumor promoters mezerein and phorbol 12-myristate 13-acetate (PMA), but not the inactive phorbol ester analog 4alpha-PMA, caused a pronounced decrease of myelin basic protein (MBP) content and 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) activity. In contrast, myelin/oligodendrocyte protein (MOG) content was affected relatively little. Northern blot analyses showed a rapid reduction of MBP and PLP gene expression induced by mezerein, and both morphological and biochemical findings indicate a drastic loss of compact myelin. During the acute phase of demyelination, only a relatively small increase in cell death was perceptible by in situ end labeling and in situ nick translation. Basic fibroblast growth factor (bFGF) also reduced the levels of the oligodendroglial differentiation markers and enhanced the demyelinating effects of the tumor promoters. The present results suggest that PKC activation resulted in severe demyelination and partial loss of the oligodendrocyte-differentiated phenotype.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Biological Markers - chemistry
- Brain - drug effects
- Carcinogens - toxicity
- Cell Aggregation - drug effects
- Cell Differentiation - drug effects
- Cells, Cultured - drug effects
- Demyelinating Diseases - chemically induced
- Diterpenes - chemically induced
- Enzyme Activation - chemically induced
- Fibroblast Growth Factor 2 - toxicity
- Gene Expression - toxicity
- Myelin Proteins - genetics
- Oligodendroglia - drug effects
- Protein Kinase C - metabolism
- Rats - metabolism
- Terpenes - toxicity
- Tetradecanoylphorbol Acetate - toxicity
- Find related publications in this database (Keywords)
- rat brain
- oligodendrocytes
- aggregating cell cultures
- demyelination
- protein kinase C activators
- basic fibroblast growth factor
- myelin basic protein
- 2'
- '-cyclic nucleotide 3'-phosphohydrolase