Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Klade, CS; Voss, T; Krystek, E; Ahorn, H; Zatloukal, K; Pummer, K; Adolf, GR.
Identification of tumor antigens in renal cell carcinoma by serological proteome analysis.
Proteomics. 2001; 1(7):890-898
Doi: 10.1002/1615-9861(200107)1:7<890::AID-PROT890>3.0.CO;2-Z
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- Co-Autor*innen der Med Uni Graz
- Pummer Karl
- Zatloukal Kurt
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- Abstract:
- We have investigated the suitability of proteomics for identification of tumor-associated antigens. First, we compared the proteomes of nontumorous kidney and renal cell carcinoma (RCC) by two-dimensional gel electrophoresis (2-DE) and silver staining. Protein patterns were markedly different (approximately 800 spots in RCCs versus approximately 1400 spots in kidney). 2-DE immunoblotting revealed five RCC-specific spots, reproducibly reactive with RCC-patient but not healthy donor control sera. Two of these antigens were isolated by preparative 2-DE, and identified by Edman sequencing of tryptic peptides. The first antigen, smooth muscle protein 22-alpha (SM22-alpha), is an actin-binding protein of unknown function predominantly expressed in smooth muscle cells. In situ hybridization revealed that SM22-alpha is not expressed in the malignant cells but in mesenchymal cells of the tumor stroma. The second antigen represents carbonic anhydrase I (CAI), an isoform usually not expressed in kidney. Interestingly, a different isoform (CAXII) has previously been identified by serological expression cloning as an antigen overexpressed in some RCCs. In additional assays, antibodies to recombinant CAI or SM22-alpha were detected in sera from 3/11 or 5/11 RCC patients, respectively, whereas sera from 13 healthy individuals did not react. In conclusion, serological proteome analysis may be a new tool for the identification of tumor-associated antigens.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Antigens, Neoplasm - analysis
- Base Sequence - analysis
- Blotting, Northern - analysis
- Carbonic Anhydrase I - analysis
- Carcinoma, Renal Cell - chemistry
- DNA, Complementary - genetics
- DNA, Neoplasm - genetics
- Electrophoresis, Gel, Two-Dimensional - genetics
- Humans - genetics
- Kidney - chemistry
- Kidney Neoplasms - chemistry
- Microfilament Proteins - analysis
- Molecular Sequence Data - analysis
- Muscle Proteins - analysis
- Peptide Fragments - genetics
- Proteome - analysis
- RNA, Messenger - genetics
- Recombinant Proteins - genetics
- Serologic Tests - methods
- Find related publications in this database (Keywords)
- serology
- proteomics
- renal cell carcinoma
- tumor-associated antigen
- tumor stroma