Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wenisch, C; Moore, CB; Krause, R; Presterl, E; Pichna, P; Denning, DW.
Antifungal susceptibility testing of fluconazole by flow cytometry correlates with clinical outcome.
J Clin Microbiol. 2001; 39(7):2458-2462
Doi: 10.1128/JCM.39.7.2458-2462.2001
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- Führende Autor*innen der Med Uni Graz
- Wenisch Christoph
- Co-Autor*innen der Med Uni Graz
- Krause Robert
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- Abstract:
- Susceptibility testing of fungi by flow cytometry (also called fluorescence-activated cell sorting [FACS]) using vital staining with FUN-1 showed a good correlation with the standard M27-A procedure for assessing MICs. In this study we determined MICs for blood culture isolates from patients with candidemia by NCCLS M27-A and FACS methods and correlated the clinical outcome of these patients with in vitro antifungal resistance test results. A total of 24 patients with candidemia for whom one or more blood cultures were positive for a Candida sp. were included. Susceptibility testing was performed by NCCLS M27-A and FACS methods. The correlation of MICs (NCCLS M27-A and FACS) and clinical outcome was calculated. In 83% of the cases, the MICs of fluconazole determined by FACS were within 1 dilution of the MICs determined by the NCCLS M27-A method. For proposed susceptibility breakpoints, there was 100% agreement between the M27-A and FACS methods. In the FACS assay, a fluconazole MIC of <1 microg/ml was associated with cure (P < 0.001) whereas an MIC of > or =1 microg/ml was associated with death (P < 0.001). The M27-A-derived fluconazole MICs did not correlate with outcome (P = 1 and P = 0.133).
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Aged -
- Aged, 80 and over -
- Antifungal Agents - pharmacology
- Blood - microbiology
- Candida - drug effects
- Candidiasis - drug therapy
- Culture Media - drug therapy
- Drug Resistance, Microbial - drug therapy
- Female - drug therapy
- Flow Cytometry - methods
- Fluconazole - pharmacology
- Fungemia - drug therapy
- Humans - drug therapy
- Infant - drug therapy
- Male - drug therapy
- Microbial Sensitivity Tests - methods
- Middle Aged - methods
- Treatment Outcome - methods