Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Trantina-Yates, A; Weissenstein, C; Human, P; Zilla, P.
Stentless bioprosthetic heart valve research: sheep versus primate model.
Ann Thorac Surg. 2001; 71(5 Suppl):S422-S427
Doi: 10.1016%2FS0003-4975%2801%2902502-4
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- Führende Autor*innen der Med Uni Graz
- Yates Ameli
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- Abstract:
- BACKGROUND: The mild inflammatory response against stented bioprosthetic heart valves in the sheep model is often opposed by a more distinct response in failing human implants. With the emergence of stentless root prostheses with their significantly larger proportion of tissue interacting with the immune system of the host, a more relevant animal model than the sheep may be needed. METHODS: Valved, porcine aortic roots of 5 cm length were fixed in 0.2% glutaraldehyde and implanted in the upper descending aorta of Merino sheep (n = 5; 43+/-3 kg) and Chacma baboons (n = 5; 17+/-3 kg). After 6 weeks of tissue calcification, pannus outgrowth and inflammation were assessed by atomic absorption spectrophotometry, histologic damage scoring (0 to 3), image analysis, and transmission electron microscopy. RESULTS: The main difference between the two animal models was in aortic wall calcification (64.8+/-39.8 microg/mg in the sheep model versus 4.1+/-5.9 microg/mg in the primate model; p > 0.005). In both models, leaflet calcification was negligible (2.6+/-2.4 microg/mg in the sheep versus 2.5+/-1.9 microg/mg in the primate), and the overall extent of inflammation was comparable (1.2+/-0.8 versus 0.98+/-0.7; p = 0.18 in the sheep and the primate, respectively). Qualitatively, the sheep demonstrated a macrophage-dominated reaction whereas the inflammatory demarcation often resembled a granulocyte-dominated xenograft response in the primate. Pannus outgrowth was comparable in length (8.4+/-2.3 mm versus 9.1+/-4.3 mm proximally and 7.1+/-3.4 mm versus 7.4+/-5.1 mm distally, in the sheep and baboon, respectively; p > 0.05). CONCLUSIONS: Our results confirm the sheep as a significantly stronger calcification model for stentless aortic heart valves than the primate. Remaining antigenicity of porcine tissue as a result of incomplete cross-linking, however, elicits a distinctly stronger xenograft-type reaction in the primate model.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Bioprosthesis -
- Calcinosis - pathology
- Disease Models, Animal - pathology
- Equipment Failure Analysis - pathology
- Heart Valve Prosthesis - pathology
- Humans - pathology
- Middle Aged - pathology
- Papio - pathology
- Prosthesis Design - pathology
- Sheep - pathology
- Species Specificity - pathology
- Spectrophotometry, Atomic - pathology
- Stents - pathology
- Swine - pathology