Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wagner, T; Fritsch, G; Thalhammer, R; Höcker, P; Lanzer, G; Lechner, K; Geissler, K.
IL-10 increases the number of CFU-GM generated by ex vivo expansion of unmanipulated human MNCs and selected CD34+ cells.
Transfusion. 2001; 41(5):659-666
Doi: 10.1046%2Fj.1537-2995.2001.41050659.x
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Wagner Thomas
- Co-Autor*innen der Med Uni Graz
- Lanzer Gerhard
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- Abstract:
- BACKGROUND: Ex vivo expansion strategies with different cytokine combinations are currently used by several groups as a means of increasing the number of HPCs for a variety of special clinical applications. Because there is little information on the potential role of IL-10 in such ex vivo expansion models, the effect of this cytokine on the generation of myeloid progenitor cells in suspension cultures was investigated. STUDY DESIGN AND METHODS: On the basis of data from the literature and from new experiments, the combination of SCF and IL-3 at concentrations of 100 ng per mL and 100 U per mL, respectively, was chosen as the standard cocktail. The addition of IL-10 to such cultures resulted in a marked and dose-dependent potentiation of myeloid progenitor cell production. RESULTS: Using unmanipulated leukapheresis components from 13 individuals (including lymphoma and cancer patients and normal donors), the expansion multiple of CFU-GM after 14 days as compared with pre-expansion values was 9.54 +/- 2.31 times by SCF/IL-3 and 46.38 +/- 7.37 times by the combination of SCF/IL-3 and 100 ng per mL of IL-10 (p<0.001). IL-10 also potentiated CFU-GM generation from selected CD34 PBMNCs (n = 9) with an expansion of 17.22 +/- 7.04 times versus 45.67 +/- 16.78 times using the SCF/IL-3 and SCF/IL-3/IL-10 combination, respectively (p<0.05). Moreover, expansion-promoting effects of IL-10 were observed in liquid cultures containing MNCs from bone marrow (n = 4) and cord blood (n = 3), but did not reach statistical significance because of the small number of samples. CONCLUSION: These results suggest IL-10 as a useful cytokine to optimize progenitor cell-expansion strategies for clinical application.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD34 - analysis
- Hematopoietic Stem Cells - drug effects
- Humans - drug effects
- Immunophenotyping - drug effects
- Interleukin-10 - pharmacology
- Leukapheresis - pharmacology
- Leukocytes, Mononuclear - drug effects