Medizinische Universität Graz - Research portal
Selected Publication:
Speicher, MR; Gwyn Ballard, S; Ward, DC.
Karyotyping human chromosomes by combinatorial multi-fluor FISH.
Nat Genet. 1996; 12(4):368-375
Doi: 10.1038/ng0496-368
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Leading authors Med Uni Graz
- Speicher Michael
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- We have developed epifluorescence filter sets and computer software for the detection and discrimination of 27 different DNA probes hybridized simultaneously. For karyotype analysis, a pool of human chromosome painting probes, each labelled with a different fluor combination, was hybridized to metaphase chromosomes prepared from normal cells, clinical specimens, and neoplastic cell lines. Both simple and complex chromosomal rearrangements could be detected rapidly and unequivocally; many of the more complex chromosomal abnormalities could not be delineated by conventional cytogenetic banding techniques. Our data suggest that multiplex-fluorescence in situ hybridization (M-FISH) could have wide clinical utility and complement standard cytogenetics, particularly for the characterization of complex karyotypes.
- Find related publications in this database (using NLM MeSH Indexing)
- Chromosome Aberrations - genetics
- Chromosomes, Artificial, Yeast - genetics
- Chromosomes, Human - genetics
- DNA Probes - genetics
- Female - genetics
- Fluorescent Dyes - genetics
- Gene Rearrangement - genetics
- Humans - genetics
- Image Processing, Computer-Assisted - genetics
- In Situ Hybridization, Fluorescence - methods
- Karyotyping - methods
- Male - methods
- Software - methods
- Tumor Cells, Cultured - methods