Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Scheunemann, P; Hosch, SB; Witter, K; Kraus, J; Speicher, MR; Pantel, K; Izbicki, JR.
Biological relevance of occult tumor cells in a lymph node of a patient with resectable esophageal cancer
LANGENBECKS ARCH SURG 1999 : 105-108.
Doi: 10.1007/978-3-642-60133-0_22
Web of Science
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Speicher Michael
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Background: The presence of immunohistochemically detectable isolated tumor cells in lymph nodes of patients with carcinoma of the gastrointestinal tract correlates with poor prognosis. However, the biological relevance of these cells is still unclear. Further analyses of such occult tumor cells are limited because of their extremely low frequency. Methods: Tissue samples from a lymph node classified as positive by immunohistochemistry but negative by routine histopathology and the autologous primary tumor wc:re harvested for cell culture and two permant cell lines could be generated. Both cell lines were analysed using microsatellite analysis, HLA-DRB1*genotyping, FAGS, M-FISH and SCID mice xenograftassay. Results: In FAGS and M-FISH analysis, both cell lines showed overexpression of p53 and p185(erbB2), were in a hypertriploid range and showed loss of the Y chromosome. Furthermore, both cell lines displayed tumorigenic behaviour in vivo after transplantation into SCID mice. Re-establishment of tumor cell lines from different murine organs indicates the presence of micrometastases in these organs. Conclusion: These data provide first direct evidence that immunohistochemically identifiable tumor cells in lymph nodes are viable malignant tumor cells, which implies that these cells might be precursors of subsequent metastatic lesions.