Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Klein, CA; Schmidt-Kittler, O; Schardt, JA; Pantel, K; Speicher, MR; Riethmüller, G.
Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells.
Proc Natl Acad Sci U S A. 1999; 96(8):4494-4499
Doi: 10.1073/pnas.96.8.4494
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- Co-Autor*innen der Med Uni Graz
- Speicher Michael
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- Abstract:
- A PCR strategy is described for global amplification of DNA from a single eukaryotic cell that enables the comprehensive analysis of the whole genome. By comparative genomic hybridization, not only gross DNA copy number variations, such as monosomic X and trisomic 21 in single male cells and cells from Down's syndrome patients, respectively, but multiple deletions and amplifications characteristic for human tumor cells are reliably retrieved. As a model of heterogeneous cell populations exposed to selective pressure, we have studied single micrometastatic cells isolated from bone marrow of cancer patients. The observed congruent pattern of comparative genomic hybridization data, loss of heterozygosity, and mutations as detected by sequencing attests to the technique's fidelity and demonstrates its usefulness for assessing clonal evolution of genetic variants in complex populations.
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- Bone Marrow Cells - cytology
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- Chromosome Mapping - genetics
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- DNA - chemistry
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- In Situ Hybridization, Fluorescence - genetics
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- Loss of Heterozygosity - cytology
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- Nucleic Acid Hybridization - genetics
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