Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Bolzer, A; Craig, JM; Cremer, T; Speicher, MR.
A complete set of repeat-depleted, PCR-amplifiable, human chromosome-specific painting probes.
Cytogenet Cell Genet. 1999; 84(3-4):233-240
Doi: 10.1159/000015266
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- Führende Autor*innen der Med Uni Graz
- Speicher Michael
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- Abstract:
- We describe the generation of a complete set of human chromosome-specific painting probes depleted in repetitive sequences. These probes yield highly specific signals when hybridized without the addition of a blocking agent, such as Cot-1 DNA, and without probe preannealing prior to hybridization. Fluorescent intensities and signal-to-background ratios for these probes are comparable to those of untreated probes hybridized with Cot-1 DNA. We demonstrate the suitability of these probes for applications with very complex probe sets, such as multiplex-FISH.
- Find related publications in this database (using NLM MeSH Indexing)
- Binding, Competitive -
- Chromosome Painting - methods
- Chromosomes, Human - genetics
- DNA - genetics
- DNA Probes - genetics
- Genome, Human - genetics
- Humans - genetics
- Nucleic Acid Hybridization - genetics
- Polymerase Chain Reaction - genetics
- Repetitive Sequences, Nucleic Acid - genetics
- Reproducibility of Results - genetics
- Sensitivity and Specificity - genetics