Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Frank, HG; Gunawan, B; Ebeling-Stark, I; Schulten, HJ; Funayama, H; Cremer, U; Huppertz, B; Gaus, G; Kaufmann, P; Füzesi, L.
Cytogenetic and DNA-fingerprint characterization of choriocarcinoma cell lines and a trophoblast/choriocarcinoma cell hybrid.
CANCER GENET CYTOGENET 2000 116: 16-22.
Doi: 10.1016/S0165-4608(99)00107-7
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- Co-Autor*innen der Med Uni Graz
- Huppertz Berthold
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- Abstract:
- We report the successful fusion of human choriocarcinoma cells with normal human trophoblast cells to a choriocarcinoma/trophoblast hybrid. The hybrid cells ACH1P were derived from fusion of primary male trophoblast cells with the HGPRT-defective choriocarcinoma cell line AC1-1. The karyotypes of the parental choriocarcinoma cell line JEG-3, its HGPRT-defective mutant clones AC1-1, AC1-5, and AC1-9, and the choriocarcinoma/trophoblast hybrid ACH1P are presented, together with a detailed characterization of the AC1-specific chromosomal marker add(X)(q26) using conventional cytogenetic banding techniques and multiplex-fluorescence in situ hybridization (M-FISH). To our knowledge, this is the first report of a stably proliferating human cell hybrid of trophoblastic origin, providing a unique cell culture model to study trophoblast-related invasion and its underlying genetic mechanisms.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Fusion -
- Choriocarcinoma - genetics
- Chromosome Banding - genetics
- DNA Fingerprinting - genetics
- Female - genetics
- Humans - genetics
- Hybrid Cells - genetics
- In Situ Hybridization, Fluorescence - genetics
- Karyotyping - genetics
- Pregnancy - genetics
- Research Support, Non-U.S. Gov't - genetics
- Trophoblasts - genetics
- Tumor Cells, Cultured - genetics
- Uterine Neoplasms - genetics