Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Pötgens, AJ; Bolte, M; Huppertz, B; Kaufmann, P; Frank, HG.
Human trophoblast contains an intracellular protein reactive with an antibody against CD133--a novel marker for trophoblast.
PLACENTA 2001 22: 639-645.
Doi: 10.1053/plac.2001.0701
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Huppertz Berthold
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- Abstract:
- CD133 is a protein expressed on the cell membrane of a subfraction of haematopoietic stem and progenitor cells, as well as on some epithelial cells. Previously available antibodies against CD133 recognized only the glycosylated protein, localized to membrane protrusions or microvilli. Due to this, immature intracellular stages of the CD133 protein could not be visualized using these antibodies. We describe reactivity of a commercially available antibody against CD133, called AC133-2, with an intracellular protein in trophoblast. Both villous and extravillous cytotrophoblast, as well as syncytiotrophoblast were stained by AC133-2 in cryostat sections of first trimester and term placenta. Villous stroma was not stained. AC133-2 reactivity was seen in methanol-fixed primary trophoblast cells and trophoblast-derived cell lines, and was coexpressed with cytokeratin-7. CD133 messenger RNA was present in trophoblast and trophoblast-derived cell lines, but also in cells not displaying any reactivity with CD133 antibodies. AC133-2 recognized a 55-60 kDa protein on Western blots of cell extracts including trophoblast. The exact nature of this protein is not yet understood. However, AC133-2 is applicable as a positive marker for the characterization of all subtypes of trophoblast and for trophoblast cell lines.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Monoclonal - immunology
- Antibody Specificity - immunology
- Antigens, CD - immunology
- Biological Markers - analysis
- Blotting, Western - analysis
- Cell Line - analysis
- Choriocarcinoma - analysis
- Epitopes - immunology
- Female - immunology
- Glycoproteins - analysis
- Humans - analysis
- Hybrid Cells - analysis
- Immunohistochemistry - analysis
- Keratin - analysis
- Peptides - analysis
- Pregnancy - analysis
- Proteins - analysis
- RNA, Messenger - analysis
- Research Support, Non-U.S. Gov't - analysis
- Reverse Transcriptase Polymerase Chain Reaction - analysis
- Trophoblasts - chemistry
- Tumor Cells, Cultured - chemistry