Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Genser, B; Truschnig-Wilders, M; Stünzner, D; Landini, MP; Halwachs-Baumann, G.
Evaluation of five commercial enzyme immunoassays for the detection of human cytomegalovirus-specific IgM antibodies in the absence of a commercially available gold standard.
Clin Chem Lab Med. 2001; 39(1):62-70
Doi: 10.1515/CCLM.2001.014
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Baumann Gabriele
- Truschnig-Wilders Martini
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- Abstract:
- In the recent years the number of commercially available immunoassays for the detection of human cytomegalovirus (HCMV)-specific immunoglobulin M (IgM) antibodies has rapidly increased. The aim of the present study was to evaluate five commercial immunoassays for the serological diagnosis of HCMV-infection. These methods, namely the IMx CMV IgM assay, the AxSYM CMV IgM assay (both Abbott), the Gull CMV IgM, the CMV-IgM-ELA test PCS Medac and the Biotest Anti-HCMV recombinant IgM ELISA, were compared for their diagnostic effectiveness and interference with substances eventually producing cross-reactions with HCMV-IgM (Epstein-Barr-virus (EBV)-IgM, rheumatoid factor (RF)). In addition, repeated measurements on samples from kidney and heart transplant recipients with active HCMV infection were examined to compare the temporal development of the HCMV-IgM measured with the five assay systems. Since there is no commercially available gold standard, it was assumed that the true classification, of whether the patient sample is HCMV-IgM positive or negative, was unknown. Hence sensitivity and specificity were assessed based on a maximum likelihood approach using a "latent class" model. The cross-reactions were quantified by a Bayesian statistical model using prior information for the expected prevalences in the EBV-IgM and rheumatoid factor sample groups. The results of the study demonstrated that there are great differences in sensitivity and specificity as well as in cross-reactions with EBV-IgM and RF between the tested ELISAs.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Aged -
- Antibodies - immunology
- Blotting, Western -
- Child -
- Child, Preschool -
- Cytomegalovirus - immunology
- Cytomegalovirus Infections - diagnosis
- Enzyme-Linked Immunosorbent Assay - instrumentation Enzyme-Linked Immunosorbent Assay - methods
- Female -
- Heart Transplantation -
- Humans -
- Immunoenzyme Techniques - methods
- Immunoglobulin G - metabolism
- Immunoglobulin M - immunology
- Kidney Transplantation -
- Likelihood Functions -
- Male -
- Middle Aged -
- Models, Statistical -
- Pregnancy -
- Reproducibility of Results -
- Sensitivity and Specificity -
- Transplantation -
- Find related publications in this database (Keywords)
- cytomegalovirus
- human (HCMV)
- HCMV-IgM
- immunoassay