Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bose, P; Black, S; Kadyrov, M; Bartz, C; Shlebak, A; Regan, L; Huppertz, B.
Adverse effects of lupus anticoagulant positive blood sera on placental viability can be prevented by heparin in vitro.
AMER J OBSTET GYNECOL 2004 191: 2125-2131.
Doi: 10.1016/j.ajog.2004.05.014
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Huppertz Berthold
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- Abstract:
- OBJECTIVE: Lupus anticoagulant poses a significant risk factor for obstetric complications, whereas heparin improves live birth rates in those pregnancies. Pathophysiology of antiphospholipid antibodies on placental function involves coagulopathies and thrombosis but also dysregulated trophoblast turnover. STUDY DESIGN: With the use of placental explant cultures, we assessed the effect of lupus anticoagulant positive sera (LA + sera) on apoptosis, mitosis, and invasion of trophoblast and determined the role of unfractionated heparin in regulating these functions. RESULTS: LA + sera were associated with increased placental apoptosis (TUNEL, M30 formation, DNA laddering). LA + sera decreased villous trophoblast proliferation and reduced extravillous trophoblast invasion through matrigel. Heparin attenuated LA + sera-induced apoptosis and facilitated trophoblast invasion. CONCLUSION: Lupus anticoagulant may impair placentation by increasing apoptosis, attenuating mitosis and reducing invasion of the trophoblast. The direct effects on trophoblast viability by heparin demonstrate an alternative biologic function for this anticoagulant and raise the possibility that anomalous trophoblast development may be therapeutically regulated.
- Find related publications in this database (using NLM MeSH Indexing)
- Abortion, Spontaneous -
- Adult -
- Antibodies, Antiphospholipid - immunology
- Apoptosis - drug effects
- Blotting, Western - drug effects
- DNA Fragmentation - drug effects
- Female - drug effects
- Heparin - pharmacology
- Humans - pharmacology
- Immunohistochemistry - pharmacology
- In Situ Nick-End Labeling - pharmacology
- In Vitro - pharmacology
- Lupus Erythematosus, Systemic - blood
- Middle Aged - blood
- Mitosis - physiology
- Placenta - cytology
- Pregnancy - cytology
- Pregnancy Trimester, First - cytology
- Reference Values - cytology
- Research Support, Non-U.S. Gov't - cytology
- Sensitivity and Specificity - cytology
- Tissue Culture Techniques - cytology
- Trophoblasts - drug effects
- Find related publications in this database (Keywords)
- anticoaaulants
- apoptosis
- heparin
- trophoblast
- matrigel