Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Langer, S; Geigl, JB; Ehnle, S; Gangnus, R; Speicher, MR.
Live cell catapulting and recultivation does not change the karyotype of HCT116 tumor cells.
Cancer Genet Cytogenet. 2005; 161(2):174-177
Doi: 10.1016/j.cancergencyto.2005.01.013
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- Führende Autor*innen der Med Uni Graz
- Speicher Michael
- Co-Autor*innen der Med Uni Graz
- Geigl Jochen Bernd
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- Abstract:
- We used laser microdissection and pressure catapulting (LMPC) to isolate and subsequently recultivate cells from the colorectal cancer cell line HCT116. Cell isolation and recultivation was repeated 5 times from the preceding cell culture. The chromosomal composition of the HCT116 cells was analyzed at the beginning and after each recultivation. We did not observe any additional chromosomal structural or copy number changes during this procedure. Our data suggest that the procedure of cell isolation by LMPC and subsequent recultivation does not affect the karyotype of the respective cells. This technology will facilitate the analysis of different cell populations in a heterogeneous tumor cell population.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Culture Techniques - methods
- Cell Separation - methods
- Colorectal Neoplasms - genetics
- HCT116 Cells - genetics
- Humans - genetics
- Karyotyping - genetics
- Microdissection - genetics
- Nucleic Acid Hybridization - genetics