Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wallner, S; Hermetter, A; Mayer, B; Wascher, TC.
The alpha-amino group of L-arginine mediates its antioxidant effect.
Eur J Clin Invest. 2001; 31(2):98-102
Doi: 10.1046%2Fj.1365-2362.10.1046%2Fj.1365-2362.2001.00771.x
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Holasek Sandra Johanna
- Wascher Thomas
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- Abstract:
- Antioxidant effects may constitute part of the possible antiatherogenic effects of the amino acid L-arginine. These antioxidant properties were further characterized in a model of lipoprotein oxidation. Oxidation of lipoproteins in unfractionated human serum was continuously monitored by a fluorescent probe. The antioxidant effects of L-arginine, N-alpha-acetyl-arginine and vitamin E in combination with L-arginine were measured after initiation of free radical generation with either copper or 2,2'-azobis(2-amidinopropane) hydrochloride (AAPH). The half-time of the fast propagation rate for copper-induced lipoprotein oxidation increased after incubation with L-arginine in a dose-dependent manner (P < 0.01). N-alpha-acetyl-arginine did not show such effects. Vitamin E and L-arginine show different effects on copper-induced oxidation, the former increasing only lag-time, the latter increasing only propagation rate, and do not have reciprocal effects. In contrast to copper-induced oxidation, L-arginine increased the lag-time of AAPH-induced lipoprotein oxidation (P < 0.01), with no effect on the propagation rate at physiological concentrations. Again, N-alpha-acetyl-arginine did not show any antioxidant effects. Our experiments provide further evidence that mechanisms other than serving as a substrate for the NO-synthase could be involved in the antiatherosclerotic effect of L-arginine. In addition, our experiments clearly show, that the antioxidant effect of L-arginine is due to a chemical moiety different from that serving as the substrate for NO biosynthesis.
- Find related publications in this database (using NLM MeSH Indexing)
- Amidines - metabolism
- Antioxidants - pharmacology
- Arginine - pharmacology
- Copper - pharmacology
- Humans - pharmacology
- Lipoproteins - metabolism
- Oxidation-Reduction - metabolism
- Stereoisomerism - metabolism
- Find related publications in this database (Keywords)
- antioxidants
- atherosclerosis
- L-arginine
- lipoprotein oxidation
- nitric oxide