Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Schulter, V; Koolwijk, P; Peters, E; Frank, S; Hrzenjak, A; Graier, WF; van Hinsbergh, VW; Kostner, GM.
Impact of apolipoprotein(a) on in vitro angiogenesis.
Arterioscler Thromb Vasc Biol. 2001; 21(3):433-438
Doi: 10.1161/01.ATV.21.3.433
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- Führende Autor*innen der Med Uni Graz
- Kostner Gerhard
- Co-Autor*innen der Med Uni Graz
- Frank Sasa
- Graier Wolfgang
- Hrzenjak Andelko
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- Abstract:
- Angiostatin, which consists of the kringle I-IV domains of plasminogen and which is secreted into urine, is an efficient inhibitor of angiogenesis and tumor growth. Because N-terminal apolipoprotein(a) [apo(a)] fragments, which also contain several types of kringle IV domains, are found in urine as well, we evaluated the potential angiostatic properties of these urinary apo(a) fragments and of a recombinant form of apo(a) [r-apo(a)]. We used human microvascular endothelial cell (hMVEC)-based in vitro assays of tube formation in 3-dimensional fibrin matrixes. Purified urinary apo(a) fragments or r-apo(a) inhibited the basic fibroblast growth factor/tumor necrosis factor-alpha-induced formation of capillary-like structures. At concentrations varying from 0.2 to 10 microgram/mL, urinary apo(a) fragments inhibited tube formation by as much as 70%, whereas there was complete inhibition by r-apo(a). The highest concentrations of both inhibitors also reduced urokinase plasminogen activator production of basic fibroblast growth factor-induced hMVEC proliferation. The inhibitors had no effect on plasminogen activator inhibitor-1 expression. If our in vitro model for angiogenesis is valid for the in vivo situation as well, our data point toward the possibility that apo(a) may also be physiologically operative in modulating angiogenesis, as the concentration of free apo(a) found in humans exceeds that tested herein.
- Find related publications in this database (using NLM MeSH Indexing)
- Angiogenesis Inhibitors - pharmacology
- Apolipoproteins A - chemistry
- Cell Division - drug effects
- Cells, Cultured - drug effects
- Dose-Response Relationship, Drug - drug effects
- Endothelium, Vascular - cytology
- Fibroblast Growth Factor 2 - pharmacology
- Humans - pharmacology
- Male - pharmacology
- Peptide Fragments - pharmacology
- Plasminogen Activator Inhibitor 1 - metabolism
- Tumor Necrosis Factor-alpha - pharmacology
- Urinary Plasminogen Activator - drug effects
- Find related publications in this database (Keywords)
- Lp(a)
- endothelial cells
- kringle
- urinary fragments