Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Shahbazian, A; Schuligoi, R; Heinemann, A; Peskar, BA; Holzer, P.
Disturbance of peristalsis in the guinea-pig isolated small intestine by indomethacin, but not cyclo-oxygenase isoform-selective inhibitors.
BRIT J PHARMACOL 2001 132: 1299-1309.
Doi: 10.1038/sj.bjp.0703940
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Holzer Peter
- Co-Autor*innen der Med Uni Graz
- Heinemann Akos
- Peskar Bernhard
- Schuligoi Rufina
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- 1. Since the cyclo-oxygenase (COX) isoform-nonselective inhibitor indomethacin is known to modify intestinal motility, we analysed the effects of COX-1 and COX-2 inhibition on intestinal peristalsis. 2. Peristalsis in isolated segments of the guinea-pig small intestine was triggered by a rise of the intraluminal pressure and recorded via the pressure changes associated with peristalsis. 3. The COX-1 inhibitor SC-560, the COX-2 inhibitor NS-398 (both at 0.1 -- 1 microM) and the isoform-nonselective inhibitors flurbiprofen (0.01 - 10 microM) and piroxicam (0.1 - 50 microM) were without major influence on peristalsis, whereas indomethacin and etodolac (0.1 -- 10 microM) disturbed the regularity of peristalsis by causing nonpropulsive circular muscle contractions. 4. Radioimmunoassay measurements showed that SC-560, NS-398, indomethacin and etodolac (each at 1 microM) suppressed the release of 6-keto-prostaglandin F(1 alpha) (6-keto-PGF(1 alpha)) from the intestinal segments. 5. Reverse transcription - polymerase chain reaction tests revealed that, relative to glyceraldehyde-3 phosphate dehydrogenase ribonucleic acid, the expression of COX-1 mRNA increased by a factor of 2.0 whereas that of COX-2 mRNA rose by a factor of 7.9 during the 2 h experimental period. 6. Pharmacological experiments indicated that the action of indomethacin to disturb intestinal peristalsis was unrelated to inhibition of L-type calcium channels, adenosine triphosphate-sensitive potassium channels or phosphodiesterase type IV. 7. These results show that selective inhibition of COX-1 and COX-2 does not grossly alter peristaltic motor activity in the guinea-pig isolated small intestine and that the effect of indomethacin to disturb the regular pattern of propulsive motility in this species is unrelated to COX inhibition.
- Find related publications in this database (using NLM MeSH Indexing)
- 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid - pharmacology
- 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester - pharmacology
- 6-Ketoprostaglandin F1 alpha - biosynthesis
- Alprostadil - pharmacology
- Animals - pharmacology
- Anti-Inflammatory Agents, Non-Steroidal - pharmacology
- Arachidonate 5-Lipoxygenase - antagonists and inhibitors
- Calcium Channel Agonists - pharmacology
- Calcium Channels - drug effects
- Cyclic AMP - metabolism
- Cyclooxygenase 1 - metabolism
- Cyclooxygenase 2 - metabolism
- Cyclooxygenase 2 Inhibitors - metabolism
- Cyclooxygenase Inhibitors - pharmacology
- Dose-Response Relationship, Drug - pharmacology
- Female - pharmacology
- Gastrointestinal Motility - physiology
- Guinea Pigs - physiology
- Indomethacin - pharmacology
- Intestine, Small - drug effects
- Isoenzymes - antagonists and inhibitors
- Male - antagonists and inhibitors
- Peristalsis - drug effects
- Potassium Channels - drug effects
- Prostaglandin-Endoperoxide Synthases - genetics
- RNA, Messenger - biosynthesis
- Receptors, Thromboxane - antagonists and inhibitors
- Research Support, Non-U.S. Gov't - antagonists and inhibitors
- Vasoconstrictor Agents - pharmacology
- Find related publications in this database (Keywords)
- intestinal peristalsis
- cyclo-oxygenase
- prostaglandins
- cyclo-oxygenase inhibitors
- indomethacin
- etodolac
- flurbiprofen
- piroxicam
- SC-560
- NS-398