Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Oettl, K; Greilberger, J; Reibnegger, C.
Dihydroneopterin and the generation of superoxide from iron ions
PTERIDINES 2000 11: 60-63.
Doi: 10.1515/pteridines.2000.11.2.60
Web of Science
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- Führende Autor*innen der Med Uni Graz
- Öttl Karl
- Co-Autor*innen der Med Uni Graz
- Greilberger Joachim
- Reibnegger Gilbert
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- Abstract:
- We describe the impact of dihydroneopterin on the formation of superoxide radicals. As radical source ferrous iron in air-saturated aequeous solution was used. Detection of superoxide was achieved using reduction of nitroblue tetrazolium to formazan. Dihydroneopterin decreases formazan formation when high concentrations of ferrous iron are used which induce a rapid superoxide formation within minutes. On the other hand, when low concentrations of ferrous iron are used, addition of dihydroneopterin to the assay mixture leads to an increase in formazan formation for several hours of incubation. We therefore conclude that the radical promoting activity of dihydroneopterin is physiologically more relevant compared to its radical scavenging properties.
- Find related publications in this database (Keywords)
- oxygen species
- nitroblue tetrazolium
- formazan
- iron