Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Mauschitz, R; Cervar, M; Hahn, T; Pürstner, P; Desoye, G.
Self-regulation of the endothelin receptor system in choriocarcinoma cells.
Biochim Biophys Acta. 2000; 1502(2):224-234
Doi: 10.1016/S0925-4439(00)00049-1
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- Führende Autor*innen der Med Uni Graz
- Desoye Gernot
- Mauschitz Renate
- Co-Autor*innen der Med Uni Graz
- Cervar-Zivkovic Mila
- Hahn Tom
- Pürstner Peter
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- Abstract:
- The human trophoblast secretes endothelin-1 (ET-1) and expresses ET receptors. The present study tested whether the transformed BeWo, JAR and JEG-3 choriocarcinoma cells: (1) secrete endothelin-1 (ET-1); (2) express both ET-A and ET-B receptor subtypes; and (3) have the potential to allow for autologous regulation of ET-receptor proteins. The cells were cultured for 24/48 h with or without 10% FCS and, in experiments on receptor regulation, with ET-1 (5-20 nM and 10 microM). ET-1 secretion was measured by RIA and receptor levels by immunoblotting. All cell types secreted ET-1 albeit at different levels and sensitivity to FCS. All cell lines expressed both ET-A (JEG-3>BeWo=JAR) and ET-B (JEG-3=JAR>BeWo) receptor subtypes, which could be up- and downregulated depending on ET-1 concentration, culture time and FCS presence. It is concluded that BeWo, JAR and JEG-3 choriocarcinoma cells secrete ET-1 and express both ET-A and ET-B receptor subtypes. The receptor levels can be regulated by ET-1. This provides the molecular basis for an autocrine system with the potential of autologous regulation of yet unidentified ET-1-induced functions.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibody Specificity -
- Cell Cycle -
- Choriocarcinoma - metabolism
- Endothelin-1 - secretion
- Female - secretion
- Humans - secretion
- Pregnancy - secretion
- Receptor, Endothelin A - secretion
- Receptor, Endothelin B - secretion
- Receptors, Endothelin - immunology
- Tumor Cells, Cultured - immunology
- Uterine Neoplasms - metabolism
- Find related publications in this database (Keywords)
- endothelin-1
- receptor
- regulation
- secretion
- choriocarcinoma