Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Egger, G; Pfragner, R; Siegl, V; Zatloukal, K; Glasner, A; Bader, A; Steindorfer, P.
The affinity of MCF7 breast cancer cells to hyaluronan substrates of different molecular weight and concentrations in an in vitro model.
Int J Oncol. 2000; 17(5):1019-1023
Doi: 10.3892/ijo.17.5.1019
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- Führende Autor*innen der Med Uni Graz
- Egger Gerd
- Co-Autor*innen der Med Uni Graz
- Bader Arnim
- Pfragner Roswitha
- Siegl Veronika
- Zatloukal Kurt
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- Abstract:
- The affinity of MCF7 breast cancer cells to hyaluronan (HA) was investigated in an in vitro model. The cells form a tightly adhering monolayer on native HA with a concentration of 5 mg/ml. On native HA at higher concentrations the cells reduce their adhesion to the substrate in favor of increased intercellular bonds, resulting in a cluster-like aggregate that tends to detach from the substrate. Aggregate formation is accomplished after 12 h incubation. The phenomenon is independent of the CD44 receptor. Degradation of native HA by hyaluronidase abolishes aggregate formation even at high HA concentrations in favor of formation of a firmly adhering monolayer. This model may help to understand tumor spread on HA tissue structures and may explain therapy successes with hyaluronidase in tumor patients.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenocarcinoma - metabolism
- Breast Neoplasms - metabolism
- Carcinoma, Ductal, Breast - metabolism
- Cell Adhesion - drug effects
- Cell Aggregation - drug effects
- Cells, Cultured - drug effects
- Female - drug effects
- Fibroblasts - cytology
- Humans - cytology
- Hyaluronic Acid - chemistry
- Hyaluronoglucosaminidase - pharmacology
- Microscopy, Electron, Scanning - pharmacology
- Molecular Weight - pharmacology
- Osmolar Concentration - pharmacology
- Tumor Cells, Cultured - drug effects
- Find related publications in this database (Keywords)
- breast cancer
- human
- in vitro assay
- hyaluronan
- adhesion