Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Lang, I; Hoffmann, C; Olip, H; Pabst, MA; Hahn, T; Dohr, G; Desoye, G.
Differential mitogenic responses of human macrovascular and microvascular endothelial cells to cytokines underline their phenotypic heterogeneity.
CELL PROLIFERATION 2001 34: 143-155.
Doi: 10.1046%2Fj.1365-2184.2001.00205.x
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Lang-Olip Ingrid
- Co-Autor*innen der Med Uni Graz
- Desoye Gernot
- Dohr Gottfried
- Hahn Tom
- Pabst Maria-Anna
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- A variety of growth factors promote the complex multistep process of angiogenesis. The mitogenic activity of vascular endothelial growth factors (VEGFs) and placental growth factors (PlGFs), known as cytokines acting predominantly on endothelial cells, was tested on human umbilical vein endothelial cells (HUVEC) and microvascular endothelial cells (MIEC) and compared with the potency of the universally acting basic fibroblast growth factor (FGF-2). The cells were seeded at different cell numbers and incubated with various doses of growth factors for a period of 24-72 h in culture medium +/- serum. Proliferation was determined by measuring the optical density after staining the cells with the tetrazolium salt WST-1. VEGF121 and VEGF165 increased the number of HUVEC and MIEC at low and high seeding densities various doses and incubation times. The efficiency of FGF-2 was less pronounced at high seeding densities of the cells under serum-free conditions. PlGF-1 and PlGF-2 stimulated mitogenesis on HUVEC only at low cell numbers and after a short incubation time by 125 +/- 3% and 102 +/- 5% (P < 0.001), respectively. Longer incubation times with the lower seeding density in the absence of FCS did not induce a significant stimulatory effect of the PlGFs. MIEC responded stronger to all growth factors. In particular under serum free conditions, PlGF-1 and PlGF-2 effectively stimulated cell proliferation by 247 +/- 54% (P < 0.01) and 288 +/- 40% (P < 0.05) at low cell numbers, and by 81 +/- 13% (P < 0.05) and 49 +/- 13% (P < 0.01), respectively, at high cell numbers. The addition of fetal calf serum caused a reduced proliferative response of all growth factors on both cell types related to the controls. In conclusion, MIEC and HUVEC differ in their proliferative response to VEGFs, PlGFs and FGF-2.
- Find related publications in this database (using NLM MeSH Indexing)
- Capillaries - cytology
- Cell Division - drug effects
- Cells, Cultured - drug effects
- Dose-Response Relationship, Drug - drug effects
- Endothelial Growth Factors - pharmacology
- Endothelium, Vascular - cytology
- Fibroblast Growth Factor 2 - pharmacology
- Genetic Heterogeneity - pharmacology
- Humans - pharmacology
- Lymphokines - pharmacology
- Neovascularization, Physiologic - physiology
- Phenotype - physiology
- Umbilical Veins - cytology
- Vascular Endothelial Growth Factor A - cytology
- Vascular Endothelial Growth Factors - cytology