Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Sametz, W; Hennerbichler, S; Glaser, S; Wintersteiger, R; Juan, H.
Characterization of prostanoid receptors mediating actions of the isoprostanes, 8-iso-PGE(2) and 8-iso-PGF(2alpha), in some isolated smooth muscle preparations.
Br J Pharmacol. 2000; 130(8):1903-1910
Doi: 10.1038/sj.bjp.0703522
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- Co-Autor*innen der Med Uni Graz
- Juan Heinz
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- Abstract:
- We investigated the contracting actions of the isoprostanes (isoPs), 8-iso-prostaglandin (PG) F(2alpha) and 8-iso-PGE(2), in comparison to the effects of the thromboxane (TX) A(2)-mimetic U 46619 and the traditional prostaglandin PGE(2) in the isolated rat aorta, isolated rat gastric fundus and the isolated guinea-pig ileum. U 46619 and 8-iso-PGF(2alpha) caused contractions in the rat aorta and rat gastric fundus in a concentration-dependent manner, whereas these agonists showed no effects in the guinea-pig ileum. However, 8-iso-PGE(2) and PGE(2) caused contractions in all isolated organs used. The prostanoid TP-receptor antagonist SQ 29,548 (10 nM) significantly antagonized vasoconstrictions induced by the agonists used in the rat aorta. SQ 29,548 at a final concentration of 3 microM, but not at lower concentrations, significantly inhibited contractions induced by U 46619, 8-iso-PGF(2alpha) and 8-iso-PGE(2) in the rat fundus. Responses to PGE(2) were unchanged. The prostanoid EP(1)-receptor antagonist SC 51089 (3 microM) significantly inhibited contractions induced by 8-iso-PGE(2) and PGE(2) in the rat fundus and in the guinea-pig ileum. SC 51089 had no effect on responses to any of the agonists tested. Our results show that 8-iso-PGE(2), in contrast to 8-iso-PGF(2alpha), can also cause contractions by activation of the EP(1)-receptors in the rat gastric fundus and the guinea-pig ileum. The findings of the present study do not support the existence of a unique isoP-receptor in the tissues used.
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- 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid - pharmacology
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- Aorta, Thoracic - drug effects
- Dinoprost - analogs and derivatives
- Dinoprostone - analogs and derivatives
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- Dose-Response Relationship, Drug - pharmacology
- F2-Isoprostanes - pharmacology
- Female - pharmacology
- Gastric Fundus - drug effects
- Guinea Pigs - drug effects
- Hydrazines - pharmacology
- Ileum - drug effects
- Isoprostanes - drug effects
- Muscle Contraction - drug effects
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- Oxazepines - pharmacology
- Prostaglandin Antagonists - pharmacology
- Rats - pharmacology
- Rats, Sprague-Dawley - pharmacology
- Receptors, Prostaglandin - antagonists and inhibitors
- Receptors, Prostaglandin E - antagonists and inhibitors
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- Find related publications in this database (Keywords)
- 8-iso-PGE(2)
- 8-iso-PGF(2 alpha)
- TP-receptor antagonist SQ 29
- 48
- EP1-receptor antagonist SC 51089
- rat aorta
- rat gastric fundus
- guinea-pig ileum