Medizinische Universität Graz - Research portal
Selected Publication:
Barth, S; Kleinhappl, B; Gutschi, A; Jelovcan, S; Marth, E.
In vitro cytokine mRNA expression in normal human peripheral blood mononuclear cells.
Inflamm Res. 2000; 49(6):266-274
Doi: 10.1007%2Fs000110050591
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Barth Sonja
- Co-authors Med Uni Graz
- Kleinhappl Barbara
- Marth Egon
- Zauner Andrea
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- Abstract:
- OBJECTIVE AND DESIGN: Normal human peripheral blood mononuclear cells (PBMC) were analyzed for basic profiles of mRNA expression of distinct genes during incubation in a standard cell culture system. MATERIAL: Human PBMC from healthy adult blood donors. METHODS: Steady-state mRNA expression was measured using a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay. RESULTS: Shortly after isolation and cell seeding, mRNA levels of monocyte-derived cytokines (IL-1alpha, IL-6, and TNF-alpha) were significantly increased, whereas lymphocyte-derived cytokines (IL-4, IFN-gamma) were not affected. Expression levels of monokines returned to basal within 24 h. At later stages of culture, the mRNA levels of all genes studied gradually increased and were significantly elevated after 96 h of incubation. CONCLUSIONS: Monokine and lymphokine mRNAs respond differently to cell culture even under control conditions. With regard to the enhanced mRNA expression of distinct cytokines in the early stages of culture, human PBMC should not be used for gene expression studies in vitro within 24 h of isolation.
- Find related publications in this database (using NLM MeSH Indexing)
- Actins - genetics
- Adult - genetics
- Cells, Cultured - genetics
- Cytokines - genetics
- Humans - genetics
- Intercellular Adhesion Molecule-1 - genetics
- L-Lactate Dehydrogenase - secretion
- Leukocytes, Mononuclear - metabolism
- RNA, Messenger - analysis
- Research Support, Non-U.S. Gov't - analysis
- Reverse Transcriptase Polymerase Chain Reaction - analysis
- Ribosomal Proteins - genetics
- Find related publications in this database (Keywords)
- human PBMC
- cell culture
- cytokines
- mRNA expression
- RT-PCR