Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Schwinger, W; Urban, C; Lackner, H; Kerbl, R; Benesch, M; Dornbusch, HJ; Sovinz, P; Schauenstein, K; Schumm, M; Handgretinger, R.
Unrelated peripheral blood stem cell transplantation with 'megadoses' of purified CD34+ cells in three children with refractory severe aplastic anemia.
Bone Marrow Transplant. 2000; 25(5):513-517
Doi: 10.1038/sj.bmt.1702202
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- Führende Autor*innen der Med Uni Graz
- Schwinger Wolfgang
- Co-Autor*innen der Med Uni Graz
- Benesch Martin
- Dornbusch Hans Jürgen
- Kerbl Reinhold
- Lackner Herwig
- Ritter-Sovinz Petra
- Schauenstein Konrad
- Urban Ernst-Christian
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- Abstract:
- Three children with refractory severe aplastic anemia were transfused with high numbers of unrelated matched (n = 2) or C-locus haploidentical mismatched (n = 1) CD34-selected peripheral blood stem cells in the absence of an HLA-identical family donor. Two leukaphereses of the donors yielded a median number of 10.1 x 10(10) nucleated cells (range 9.7-15.4) with a median number of 9.89 x 10(8) CD34+ cells (range 7.46-26.1) and a median percentage of CD34+cells of 0.98% (range 0.77-1.7). After positive selection by magnetic cell sorting the patients received a median of 14.3 x 10(6) CD34+ cells/kg (range 11.7-24.3) and of 1.3 x 10(4) CD3+ cells/kg (range 0.57-5.8). Median time to ANC >/=0.5 x 10(9)/l was 7 days (range 7-12) and to platelets >/=20 x 10(9)/l 13 days (range 13-27). Chimerism analysis of peripheral blood after transplantation revealed permanent 100% donor hematopoiesis in all patients. The patient with the C-locus haploidentical mismatch presented with acute GVHD (grade III-IV) of the skin, liver and lower gastrointestinal tract (onset day +40) and died despite intensive immunosuppressive treatment on day +238. The two survivors developed lymphopoietic recovery of B and T lymphocytes within 3 months after transplantation. To our knowledge this experience represents the first report of transplantation with unrelated CD34+ enriched peripheral blood stem cell in children with refractory severe aplastic anemia. Bone Marrow Transplantation (2000) 25, 513-517.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Anemia, Aplastic - complications
- Antigens, CD - analysis
- Antigens, CD34 - blood
- CD4-CD8 Ratio - blood
- Child - blood
- Female - blood
- Graft Survival - blood
- Graft vs Host Disease - etiology
- Hematopoietic Stem Cell Transplantation - adverse effects
- Humans - adverse effects
- Leukapheresis - methods
- Lymphocyte Count - methods
- Male - methods
- Stem Cells - immunology
- Time Factors - immunology
- Tissue Donors - immunology
- Transplantation Chimera - immunology
- Transplantation, Homologous - immunology
- Find related publications in this database (Keywords)
- MACS
- CD-34 selection
- unrelated donor transplantation
- severe aplastic anemia