Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wolf, P; Maier, H; Müllegger, RR; Chadwick, CA; Hofmann-Wellenhof, R; Soyer, HP; Hofer, A; Smolle, J; Horn, M; Cerroni, L; Yarosh, D; Klein, J; Bucana, C; Dunner, K; Potten, CS; Hönigsmann, H; Kerl, H; Kripke, ML.
Topical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha.
J Invest Dermatol. 2000; 114(1):149-156
Doi: 10.1046/j.1523-1747.2000.00839.x
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- Führende Autor*innen der Med Uni Graz
- Wolf Peter
- Co-Autor*innen der Med Uni Graz
- Cerroni Lorenzo
- Hofer Angelika
- Hofmann-Wellenhof Rainer
- Horn Michael
- Kerl Helmut
- Muellegger Robert
- Smolle Josef
- Soyer Hans Peter
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- Abstract:
- Exposing human skin to ultraviolet radiation causes DNA damage, sunburn, immune alterations, and eventually, skin cancer. We wished to determine whether liposomes containing a DNA repair enzyme could prevent any of the acute effects of irradiation when applied after ultraviolet exposure. Fifteen human patients with a prior history of skin cancer were exposed to two minimal erythema doses of ultraviolet radiation on their buttock skin. Liposomes containing T4 endonuclease V or heat-inactivated enzyme were applied immediately and at 2, 4, and 5 h after ultraviolet irradiation. Transmission electron microscopy after anti-T4 endonuclease V-staining and immunogold labeling on biopsies taken at 6 h after ultraviolet exposure revealed that the enzyme was present within cells in the skin. Immunohistochemical DNA damage studies suggested a trend toward improved DNA repair at the active T4 endonuclease V liposome-treated test sites. Although the active T4 endonuclease V liposomes did not significantly affect the ultraviolet-induced erythema response and microscopic sunburn cell formation, they nearly completely prevented ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha RNA message and of interleukin-10 protein. These studies demonstrate that liposomes can be used for topical intracellular delivery of small proteins to human skin and suggest that liposomes containing DNA repair enzymes may provide a new avenue for photoprotection against some forms of ultraviolet-induced skin damage.
- Find related publications in this database (using NLM MeSH Indexing)
- Administration, Topical -
- Adult -
- Aged -
- DNA Ligases - administration and dosage
- DNA Repair - drug effects
- Deoxyribonuclease (Pyrimidine Dimer) - drug effects
- Drug Carriers - drug effects
- Endodeoxyribonucleases - administration and dosage
- Female - administration and dosage
- Humans - administration and dosage
- Interleukin-10 - metabolism
- Keratinocytes - enzymology
- Langerhans Cells - enzymology
- Liposomes - enzymology
- Male - enzymology
- Microscopy, Electron - enzymology
- Middle Aged - enzymology
- Radiation-Protective Agents - administration and dosage
- Skin - drug effects
- Time Factors - drug effects
- Tumor Necrosis Factor-alpha - metabolism
- Ultraviolet Rays - metabolism
- Up-Regulation - drug effects
- Viral Proteins - drug effects
- Find related publications in this database (Keywords)
- cyclobutane pyrimidine dimers
- cytokines
- DNA damage
- DNA repair enzyme
- photoprotection