Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Zabe, M; Feltzer, RE; Malle, E; Sattler, W; Dean, WL.
Effects of hypochlorite-modified low-density and high-density lipoproteins on intracellular Ca2+ and plasma membrane Ca(2+)-ATPase activity of human platelets.
Cell Calcium. 1999; 26(6):281-287
Doi: 10.1054/ceca.1999.0081
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- Co-Autor*innen der Med Uni Graz
- Malle Ernst
- Sattler Wolfgang
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- Abstract:
- The presence of hypochlorite-modified lipoproteins in atherosclerotic lesions suggests that HOCl, a naturally occurring oxidant formed by the myeloperoxidase-catalyzed reaction of H2O2 and Cl-, is a candidate for generation of modified lipoproteins in vivo. We have previously demonstrated that Cu(2+)-oxidized LDL inhibits platelet plasma membrane Ca(2+)-ATPase (PMCA) in isolated membranes and causes an increase in cytosolic Ca2+ in resting whole platelets. However, Cu(2+)-oxidized LDL may not be identical in structure and function to the physiologically modified lipoprotein. Since platelet function may be affected by native and modified lipoproteins, the effect of HOCl-modified LDL and HDL3 on platelet PMCA and on the free intracellular Ca2+ concentration ([Ca2+]i) of whole platelets has been investigated. We demonstrate that in contrast to Cu(2+)-oxidized LDL, HOCl-modified LDL and HDL3 stimulate platelet PMCA activity in isolated membranes and that this effect results in a decrease of [Ca2+]i in vivo. Thus, HOCl-oxidation produces modified lipoproteins with the potential for altering platelet function and with properties different from those of the Cu(2+)-oxidized counterparts.
- Find related publications in this database (using NLM MeSH Indexing)
- Blood Platelets - drug effects
- Calcium - metabolism
- Calcium-Transporting ATPases - drug effects
- Cell Membrane - drug effects
- Cytoplasm - drug effects
- Fura-2 - analysis
- Humans - analysis
- Hypochlorous Acid - chemistry
- Lipoproteins, HDL - chemistry