Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Dandachi, N; Balic, M; Stanzer, S; Halm, M; Resel, M; Hinterleitner, TA; Samonigg, H; Bauernhofer, T.
Critical evaluation of real-time reverse transcriptase-polymerase chain reaction for the quantitative detection of cytokeratin 20 mRNA in colorectal cancer patients.
J Mol Diagn. 2005; 7(5):631-637
Doi: 10.1016/S1525-1578(10)60597-1
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Dandachi Nadia
- Co-Autor*innen der Med Uni Graz
- Balic Marija
- Bauernhofer Thomas
- Halm Michael
- Hinterleitner Thomas
- Resel Margit
- Samonigg Hellmut
- Stanzer Stefanie
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- We evaluated the usefulness of cytokeratin 20 (CK20) mRNA expression in the quantitative detection of circulating tumor cells in the blood of patients with colorectal cancer (CRC). Blood samples from healthy volunteers (HVs; n = 37), patients with localized (n = 42) and metastatic colorectal cancer (n = 40), and patients with chronic inflammatory bowel disease (CID; n = 15) were examined. After immunomagnetic enrichment using microbeads against human epithelial antigen, total RNA was extracted, reverse transcribed, and analyzed by real-time reverse transcriptase-polymerase chain reaction using the LightCycler instrument. CK20 expression in peripheral blood was found in 46 of 82 (56%) patients with CRC, 8 of 37 (22%) HVs, and 9 of 15 (60%) patients with CID. Levels of CK20 mRNA were significantly higher in blood samples from CRC patients (median 681) than in blood samples from HVs (median 0) (P = 0.001), whereas no difference could be detected between patients with CRC and CID. Although the present technique could not distinguish CRC from CID, the method warrants further efforts to improve sample preparation and tumor cell enrichment, which may render real-time CK20 reverse transcriptase-polymerase chain reaction a feasible technique in identifying circulating tumor cells in peripheral blood of cancer patients.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Aged, 80 and over -
- Case-Control Studies -
- Colorectal Neoplasms - genetics
- Female -
- HT29 Cells -
- Health -
- Humans -
- Hydroxymethylbilane Synthase - genetics
- Inflammatory Bowel Diseases - genetics
- Keratin-20 -
- Keratins - blood
- Keratins - genetics
- Male -
- Middle Aged -
- RNA, Messenger - analysis
- RNA, Messenger - genetics
- ROC Curve -
- Reference Standards -
- Reverse Transcriptase Polymerase Chain Reaction - standards
- Sensitivity and Specificity -