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Selected Publication:
Groschner, K; Hingel, S; Lintschinger, B; Balzer, M; Romanin, C; Zhu, X; Schreibmayer, W.
Trp proteins form store-operated cation channels in human vascular endothelial cells.
FEBS Lett. 1998; 437(1-2):101-106
Doi: 10.1016/S0014-5793(98)01212-5
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- Leading authors Med Uni Graz
- Groschner Klaus
- Co-authors Med Uni Graz
- Schreibmayer Wolfgang
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- Abstract:
- Members of the Trp protein family have been suggested as the structural basis of store-operated cation conductances. With this study, we provide evidence for the expression of three isoforms of Trp (hTrp1, 3 and 4) in human umbilical vein endothelial cells (HUVEC). The role of Trp proteins in store regulation of endothelial membrane conductances was tested by expression of an N-terminal fragment of hTrp3 (N-TRP) which exerts a dominant negative effect on Trp channel function presumably due to suppression of channel assembly. Depletion of intracellular Ca2+ stores with IP3 (100 microM) or thapsigargin (100 nM) induced a substantial cation conductance in sham-transfected HUVEC as well as in HUVEC transfected with hTrp3. In contrast, HUVEC transfected with N-TRP failed to exhibit store-operated currents. Our results suggest the involvement of Trp related proteins in the store-operated cation conductance of human vascular endothelial cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Calcium Channels - genetics Calcium Channels - metabolism
- Cells, Cultured -
- Endothelium, Vascular - metabolism
- Humans -
- Patch-Clamp Techniques -
- TRPC Cation Channels -
- Thapsigargin - pharmacology
- Transfection -
- Umbilical Veins -
- Find related publications in this database (Keywords)
- Vascular Endothelium
- Store-Operated Cation Channel
- Trp Channel
- Protein
- Ip3