Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Rinner, I; Eren, R; Skreiner, E; Kukulansky, T; Kasai, M; Hirokawa, K; Globerson, A; Schauenstein, K.
Thymocyte-directed enhancement of apoptosis via soluble factor(s) derived from a cortical and a medullary thymic epithelial cell line.
Cell Tissue Res. 1996; 284(2):327-330
Doi: 10.1007%2Fs004410050592
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Schauenstein Konrad
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Apoptosis of murine thymocytes was examined either in intact fetal thymus lobes or in thymus cell suspensions, both cultured alone or in the presence of either a cortical (TEC 1.4) or a medullary (TEC 2.3) thymic epithelial cell line. Both TECs induced a pronounced increase of apoptosis in 24-h cultivated single thymus cell suspensions but not in spleen or bone marrow cell cultures. Co-culture of thymocytes with murine fibroblasts did not enhance apoptosis of the thymus cells. A similar enhancement of thymocyte apoptosis was observed with dialysed culture supernatants derived from both TEC lines, the active component(s) having a molecular weight of > 30 kDa. In contrast, the cortical TEC 1.4 had a pronounced apoptosis inducing effect on intact fetal thymus lobes cultivated for six days, whereas the medullary TEC 2.3 had only a marginal influence. TEC 1.4 also induced a significant alteration in the ratio of CD4+CD8+ to CD4-CD8- cells. It is concluded that both the cortical and medullary epithelial cell lines are able to induce thymocyte apoptosis but that a large proportion of the cells within the intact thymus stroma is refractory to the respective signal(s) of the medullary epithelial cell line.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Apoptosis - drug effects
- Cell Aging - physiology
- Cell Line - cytology
- Culture Media, Conditioned - chemistry
- Epithelial Cells - chemistry
- Fetus - cytology
- Flow Cytometry - cytology
- Lymphocytes - cytology
- Mice - cytology
- Mice, Inbred C57BL - cytology
- Solubility - cytology
- Thymus Gland - cytology
- Find related publications in this database (Keywords)
- Thymus
- Thymic Epithelial Cells
- Cell Culture
- Organ Culture
- Apoptosis
- Mouse (C57BL/6J)