Medizinische Universität Graz - Research portal
Selected Publication:
Hacker, GW; Graf, AH; Hauser-Kronberger, C; Wirnsberger, G; Schiechl, A; Bernatzky, G; Wittauer, U; Su, HC; Adam, H; Thurner, J.
Application of silver acetate autometallography and gold-silver staining methods for in situ DNA hybridization.
Chin Med J (Engl). 1993; 106(2):83-92
Web of Science
PubMed
- Co-authors Med Uni Graz
- Wirnsberger Gerhard
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- Abstract:
- In situ hybridization using biotinylated DNA probes has become an important tool in histopathology. It is well known that the sensitivity of the methods used to demonstrate viral DNA in formalin-fixed and paraffin-embedded specimen depends strongly on the detection system used. In the present study, an optimized in situ DNA hybridization protocol was combined with four different approaches of gold-silver staining methods. For silver enhancement, the recently described method of silver acetate autometallography, a technique allowing highly efficient development without the necessity of dark room illumination has been used. The most efficient detection method found in our experiments was the use of gold-adsorbed anti-biotin antibodies with subsequent silver enhancement. This staining procedure can be completed in 5 hours including hybridization and is a highly sensitive alternative to peroxidase and alkaline phosphatase detection systems.
- Find related publications in this database (using NLM MeSH Indexing)
- Cytomegalovirus - genetics
- DNA Probes - genetics
- DNA, Viral - analysis
- Gold - analysis
- Humans - analysis
- Immunohistochemistry - analysis
- In Situ Hybridization - analysis
- Papillomaviridae - genetics
- Silver Staining - methods