Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Schaider, H; Haberkorn, U; Berger, MR; Oberdorfer, F; Morr, I; van Kaick, G.
Application of alpha-aminoisobutyric acid, L-methionine, thymidine and 2-fluoro-2-deoxy-D-glucose to monitor effects of chemotherapy in a human colon carcinoma cell line.
Eur J Nucl Med. 1996; 23(1):55-60
Doi: 10.1007/BF01736990
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Schaider Helmut
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Up to 4 h after treatment of human SW 707 colon carcinoma cells with the antineoplastic drug 4-amino-N-(2'-aminophenyl)-benzamide (GOE 1734, dinaline), the effects of tumour cell metabolism and proliferation were examined in vitro. Four tracers which can be labelled with isotopes suitable for positron emission tomography (PET) were used for this purpose: alpha-aminoisobutyric acid (AIB) and methionine to study changes in amino acid transport and protein synthesis, thymidine to observe changes in tumour proliferation and 2-fluoro-2-deoxy-D-glucose (FDG) to estimate glucose metabolism. Dinaline showed an inhibition of the sodium-dependent and -independent uptake of AIB. The methionine uptake was found to increase shortly after therapy. Thymidine incorporation into DNA was impaired and the FDG uptake showed a maximally 2.2-fold enhancement. Inhibition of AIB uptake suggests changes in amino acid transport, whereas increased uptake of methionine and FDG points to an enhancement of protein synthesis and glycolysis caused by repair mechanisms. The cytostatic and antiproliferative effect of dinaline, observed in cell growth curves, could be demonstrated by the impaired thymidine incorporation into DNA. This study demonstrates that in vitro screening with radiotracers suitable for PET can help to clarify effects of new antineoplastic substances on tumour cell metabolism. These data may be applied to choose the appropriate time schedule for monitoring therapeutic effects on tumour tissue.
- Find related publications in this database (using NLM MeSH Indexing)
- Aminoisobutyric Acids - diagnostic use
- Antineoplastic Agents - therapeutic use
- Carbon Radioisotopes - diagnostic use
- Colonic Neoplasms - drug therapy
- Deoxyglucose - analogs and derivatives
- Dose-Response Relationship, Drug - analogs and derivatives
- Drug Screening Assays, Antitumor - analogs and derivatives
- Fluorodeoxyglucose F18 - analogs and derivatives
- Humans - analogs and derivatives
- Methionine - diagnostic use
- Phenylenediamines - therapeutic use
- Thymidine - diagnostic use
- Tomography, Emission-Computed - diagnostic use
- Tritium - diagnostic use
- Tumor Cells, Cultured - diagnostic use