Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
FURST, W; HALLSTROM, S.
Simultaneous determination of myocardial nucleotides, nucleosides, purine bases and creatine phosphate by ion-pair high-performance liquid chromatography.
J CHROMATOGR-BIOMED APPL. 1992; 578(1): 39-44.
Doi: 10.1016/0378-4347(92)80222-C
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- Co-Autor*innen der Med Uni Graz
- Hallström Seth
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- Abstract:
- An ion-pair reversed-phase high-performance liquid chromatographic method is described for the separation and quantification of myocardial nucleotides, nucleosides, their metabolites and creatine phosphate-related compounds in a single run. Separation of a standard mixture containing 21 compounds was achieved on a 5-microns Hypersil ODS column with a 5-min isocratic elution (buffer: 0.1 M NaH2PO4, pH 5.5, containing 5.9 mM tetrabutylammonium hydrogen-sulphate) followed by a slow linear gradient to 17% acetonitrile. The method was applied to extracts of freeze-clamped rat heart tissue samples as well as to extracts of neonatal rat heart cardiomyocytes, and it provided good resolution of high-energy phosphates, including creatine phosphate, as well as of their degradation products.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Chromatography, High Pressure Liquid - methods
- Myocardium - chemistry
- Phosphocreatine - analysis
- Purine Nucleosides - analysis
- Purine Nucleotides - analysis
- Purines - analysis
- Rats - analysis
- Rats, Inbred Strains - analysis
- Tissue Extracts - chemistry