Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Gasser, H; Hallström, S; Redl, H; Schlag, G.
Oxidatively modified plasma phospholipids containing reactive carbonyl functions measured by HPLC: evidence for phosphatidylcholine-bound aldehydes in plasma of burn patients.
Free Radic Res. 1995; 22(4):327-336
Doi: 10.3109/10715769509145645
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- Co-Autor*innen der Med Uni Graz
- Hallström Seth
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- Abstract:
- A HPLC method has been developed to measure phosphatidylcholine (PC) containing reactive carbonyl functions in the sn-acyl residue in order to study processes in which such reactive carbonyls can be formed due to e.g. oxidative fragmentation. The method has been applied to determine PC-bound carbonyls as 2,4-dinitrophenylhydrazones (DNPH) in plasma of burn patients. Plasma from healthy volunteers served as controls. Additionally, in vitro oxidation experiments (A: plasma, buffer diluted; B: plasma + iron-EDTA complex and C: plasma + iron-EDTA complex + H2O2) have been performed to obtain and to identify 2,4-dinitrophenylhydrazine derivatizable carbonyl functions in plasma PC. Both, the PC-aldehydes and PC-aldehyde DNPH derivatives were cleavable with phospholipase C. Quantification was based on thin-layer chromatography purified soybean phosphatidylcholine, which was identically oxidized and derivatized as the plasma lipids in vitro.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aldehydes - blood
- Burns - metabolism
- Chromatography, High Pressure Liquid -
- Edetic Acid - metabolism
- Female -
- Humans -
- Hydrogen Peroxide - metabolism
- Iron Chelating Agents - metabolism
- Male -
- Middle Aged -
- Oxidation-Reduction -
- Phenylhydrazines - metabolism
- Phosphatidylcholines - blood Phosphatidylcholines - metabolism
- Soybeans -
- Spectrophotometry -
- Find related publications in this database (Keywords)
- PHOSPHOLIPID-BOUND ALDEHYDES
- PHOSPHATIDYLCHOLINE
- OXIDIZED PHOSPHATIDYLCHOLINE
- LIPID PEROXIDATION
- CATION-EXCHANGE HPLC
- BURN PATIENTS