Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Podesser, BK; Hallström, S; Schima, H; Huber, L; Weisser, J; Kröner, A; Fürst, W; Wolner, E.
The erythrocyte-perfused "working heart" model: hemodynamic and metabolic performance in comparison to crystalloid perfused hearts.
J Pharmacol Toxicol Methods. 1999; 41(1):9-15
Doi: 10.1016/S1056-8719(99)00018-0
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- Co-Autor*innen der Med Uni Graz
- Hallström Seth
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- Abstract:
- A brief period of ischemia was used to evaluate an erythrocyte-enriched Krebs-Henseleit (KH) buffer (n=8) compared to KH only (n=8) in an isolated working rabbit heart. Experimental protocol was as follows: preischemic baseline, 5 min of global ischemia followed by 45 min of reperfusion. Preischemic heart rate was identical, coronary flow was significantly lower (2.7 versus 5.6 mL/min/g wet wt, p<0.01), the other hemodynamic and biochemical values were significantly higher in erythrocyte-perfused hearts: aortic flow 23.5 versus 12.0, p<0.01; cardiac output 26.2 versus 17.6, p<0.01; all in mL/min/g wet wt; dp/dt max 1286 versus 997 mmHg/s, p<0.01; myocardial oxygen consumption 3.5 versus 2.3 micromol/min/g wet wt, p<0.05. During early reperfusion, in the erythrocyte-perfused hearts, coronary flow further increased (p<0.003), the other hemodynamic parameters returned to baseline values in both groups. High-energy phosphates showed significantly higher values (ATP 2.0+/-0.1 versus 1.3+/-0.1, p<0.05; CrP 2.0+/-0.2 versus 1.6+/-0.1, p<0.05 all in micromol/g wct wt), water content was significantly lower (81% versus 74%, p<0.05) in erythrocyte-perfused hearts. It can be concluded that the erythrocyte-perfused working heart model provides excellent oxygenation, leading to superior hemodynamic and metabolic performance. Additionally, in the erythrocyte-perfused hearts preservation of coronary flow reserve underlines the physiological competency of this preparation.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenine Nucleotides - metabolism
- Animals -
- Body Water - metabolism
- Buffers -
- Coronary Vessels - physiology
- Erythrocytes - physiology
- Heart - physiology
- Hemodynamics - physiology
- Male -
- Models, Cardiovascular -
- Myocardial Ischemia - metabolism
- Myocardial Reperfusion Injury - metabolism
- Myocardium - metabolism
- Oxygen Consumption -
- Perfusion - instrumentation
- Rabbits -
- Vascular Resistance - physiology
- Find related publications in this database (Keywords)
- working heart preparation
- physiology
- erythrocyte
- coronary flow reserve