Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Kos, T; Moser, P; Yilmatz, N; Mayer, G; Pacher, R; Hallstrom, S.
High-performance liquid chromatographic determination of p-aminohippuric acid and iothalamate in human serum and urine: comparison of two sample preparation methods.
J Chromatogr B Biomed Sci Appl. 2000; 740(1):81-85
Doi: 10.1016/S0378-4347(00)00024-4
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- Co-Autor*innen der Med Uni Graz
- Hallström Seth
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- Abstract:
- A high-performance liquid chromatography method applied to determine p-aminohippuric acid (PAH) and iothalamate (IOT) in serum and urine samples of patients was evaluated according to recovery, reproducibility and linearity utilizing narrow-bore columns. The mobile phase consisted of 0.15 M sodium dihydrogenphosphate with 1.2 mM tetrabutylammonium sulphate, the pH was adjusted to pH 4.6, acetonitrile was added to a final ratio of 95:5 (v/v), the flow-rate was set at 0.3 ml/min. The separation was achieved on a ODS Hypersil column (200 x 2.1 mm I.D.). The UV detector was set at 254 nm. PAH and IOT are used for evaluation of kidney function [effective renal plasma flow (ERPF) and glomerular filtration rate (GFR)]). Under the described chromatographic conditions two sample preparation techniques, ultrafiltration and acetonitrile precipitation were compared. The results demonstrate the accuracy of both methods in evaluation of ERPF and GFR. Due to its cost-effectiveness we recommend the acetonitrile precipitation method in clinical routine.
- Find related publications in this database (using NLM MeSH Indexing)
- Chromatography, High Pressure Liquid - methods
- Humans - methods
- Iothalamic Acid - analysis
- Kidney Function Tests - analysis
- Reference Standards - analysis
- Reproducibility of Results - analysis
- p-Aminohippuric Acid - analysis
- Find related publications in this database (Keywords)
- p-aminohippuric acid
- iothalamate