Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Graier, WF; Simecek, S; Hoebel, BG; Wascher, TC; Dittrich, P; Kostner, GM.
Antioxidants prevent high-D-glucose-enhanced endothelial Ca2+/cGMP response by scavenging superoxide anions.
Eur J Pharmacol. 1997; 322(1):113-122
Doi: 10.1016%2FS0014-2999%2896%2900989-2
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Graier Wolfgang
- Co-Autor*innen der Med Uni Graz
- Kostner Gerhard
- Wascher Thomas
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Very recently we proposed that hyperactivity of endothelial Ca2+/cGMP signaling under hyperglycemic conditions is due to superoxide anion (O2-) release. The present study was designed to investigate changes in endothelial glutathione (GSH) levels in response to high D-glucose and possible prevention of the high-D-glucose-initiated changes in Ca2+/cGMP signal by antioxidants. Under hyperglycemic conditions, GSH content increased by 29% within 4 h. Co-incubation with 10 mM GSH during high-D-glucose treatment normalized the Ca2+/cGMP response associated with an increase in GSH content by 222%. Vitamin C (250 microM) markedly diminished the high-D-glucose-mediated hyperreactivity of endothelial Ca2+ entry (by 40%) and Ca2+ release (by 52%). Similar to GSH, co-incubation with vitamin E (alpha-tocopherol; 50 micrograms/ml) and probucol (50 microM) completely prevented the high-D-glucose-initiated hyperreactivity of the endothelial Ca2+/cGMP response. Vitamin E, probucol, GSH and vitamin C diminished the high-D-glucose-mediated O2- release by 78, 65, 89 and 46%, respectively. These data suggest that antioxidants prevent high-D-glucose-initiated changes in endothelial Ca2+/cGMP response by scavenging the overshoot of O2-.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antilipemic Agents - pharmacology
- Antioxidants - pharmacology
- Ascorbic Acid - pharmacology
- Calcium - metabolism
- Cells, Cultured - metabolism
- Cyclic GMP - biosynthesis
- Cytosol - metabolism
- Endothelium, Vascular - cytology
- Free Radical Scavengers - pharmacology
- Glucose - antagonists and inhibitors
- Glutathione - metabolism
- Nitric Oxide - physiology
- Probucol - pharmacology
- Superoxides - metabolism
- Swine - metabolism
- Vitamin E - pharmacology
- Find related publications in this database (Keywords)
- D-Glucose Oxidation
- Endothelial Cell
- EDRF (Endothelium-Derived Relaxing Factor)
- Glutathione
- Superoxide Dismutase
- Vitamin-C
- Vitamin-E