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Chen, Q; Esterbauer, H; Jürgens, G.
Studies on epitopes on low-density lipoprotein modified by 4-hydroxynonenal. Biochemical characterization and determination.
Biochem J. 1992; 288 ( Pt 1)(3):249-254 Doi: 10.1042/bj2880249 [OPEN ACCESS]
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Leading authors Med Uni Graz: Jürgens Günther
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Abstract:: Oxidation of human low-density lipoprotein (LDL) was found to be accompanied by the generation of various reactive aldehydes. One of them, 4-hydroxynonenal (HNE), was shown to modify LDL to a form which represents a good model of oxidized LDL (ox-LDL). In order to investigate the epitopes newly formed on HNE-modified LDL, a polyvalent antiserum to HNE-LDL [anti-(HNE-LDL)] was raised in rabbits and the non-specific components were removed with native LDL coupled to CNBr-Sepharose 4B. Competitive fluorescence immunoassay analysis showed that anti-(HNE-LDL) recognized HNE-LDL, copper-oxidized LDL, HNE-albumin and to a lower extent HNE-modified high-density lipoprotein 3 (HNE-HDL3) and ox-HDL3 but not native LDL. A certain degree of cross-reactivity of the antibody with LDLs modified by either hexanal or 2,4-heptadienal was found. No reaction was obtained with LDL labelled with malondialdehyde. From the abilities of HNE-modified poly(L-amino acids) to compete with HNE-LDL for binding to anti-(HNE-LDL), it is postulated that lysine, tyrosine, arginine and histidine are involved in the formation of HNE-derived epitopes on apolipoprotein B (apo B). Using a double-sandwich fluorescence immunoassay [capture antibody: anti-(apo B); detection antibody: anti-(HNE-LDL)] we found that the HNE-derived epitopes were expressed at a far higher degree in ox-LDL and HNE-LDL than in native LDL.
Find related publications in this database (using NLM MeSH Indexing): Aldehydes - pharmacology; Amino Acids - analysis; Antibody Specificity - analysis; Apolipoproteins B - analysis; Binding, Competitive - analysis; Epitopes - chemistry; Fluorescence - chemistry; Humans - chemistry; Immune Sera - immunology; Immunoassay - immunology; Lipoproteins, LDL - chemistry; Malondialdehyde - pharmacology; Oxidation-Reduction - pharmacology