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Schirgi-Degen, A; Beubler, E.
Significance of nitric oxide in the stimulation of intestinal fluid absorption in the rat jejunum in vivo.
Br J Pharmacol. 1995; 114(1):13-18
Doi: 10.1111/j.1476-5381.1995.tb14899.x
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- Leading authors Med Uni Graz
- Beubler Eckhard
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- Abstract:
- 1. The effects of inhibiting nitric oxide (NO)-synthase on fluid transport, mucosal cyclic GMP and cyclic AMP levels and intraluminal prostaglandin E2 (PGE2)-release were studied in a model of ligated jejunal loops of anaesthetized rats in vivo. Experiments were performed under basal conditions as well as under conditions, when net fluid secretion was induced by Escherichia coli heat stable enterotoxin a (E. coli STa) or PGE2. 2. Intravenous infusion of the NO-synthase inhibitor N omega-nitro-L-arginine methyl ester (L-NAME, 0.25-50 mg kg-1, 45 min) dose-dependently reversed net fluid absorption to net secretion, whereas infusion of D-NAME, the inactive enantiomer of L-NAME, in corresponding doses did not influence net fluid transport. N omega-nitro-L-arginine (L-NOARG, 25 mg kg-1), another NO-synthase inhibitor, also elicited net secretion of fluid. 3. L-NAME (25 mg kg-1)-induced net fluid secretion was reversed to net absorption by infusion of L-arginine (400 mg kg-1) or sodium nitroprusside (1 mg kg-1) and s.c. administration of indomethacin (10 mg kg-1). Hexamethonium (1 mg kg-1, s.c.), a ganglionic blocker and granisetron (100 micrograms kg-1, s.c.), a 5-HT3-receptor antagonist, did not influence L-NAME-induced net secretion. 4. Net fluid secretion induced by intraluminal instillation of E. coli STa (10 units ml-1) was enhanced by infusion of L-NAME (25 mg kg-1) and was inhibited by infusion of L-arginine (400 mg kg-1) and sodium nitroprusside (1 mg kg-1).(ABSTRACT TRUNCATED AT 250 WORDS)
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Arginine - analogs and derivatives
- Cyclic AMP - pharmacology
- Dinoprostone - pharmacology
- Dose-Response Relationship, Drug - pharmacology
- Female - pharmacology
- Indomethacin - pharmacology
- Intestinal Absorption - drug effects
- Jejunum - physiology
- NG-Nitroarginine Methyl Ester - physiology
- Nitric Oxide - antagonists and inhibitors
- Rats - antagonists and inhibitors
- Rats, Sprague-Dawley - antagonists and inhibitors
- Find related publications in this database (Keywords)
- Intestinal Fluid Transport
- Nitric Oxide
- Escherichia Coli Enterotoxin-Induced Secretion
- Prostaglandin E(2)
- Cyclic GMP
- N-Omega-Nitro-L-Arginine Methyl Ester (L-Name)
- L-Arginine
- Sodium Nitroprusside
- Indomethacin