Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Liang, FT; Aberer, E; Cinco, M; Gern, L; Hu, CM; Lobet, YN; Ruscio, M; Voet, PE; Weynants, VE; Philipp, MT.
Antigenic conservation of an immunodominant invariable region of the VlsE lipoprotein among European pathogenic genospecies of Borrelia burgdorferi SL.
J Infect Dis. 2000; 182(5):1455-1462
Doi: 10.1086%2F315862
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- Co-Autor*innen der Med Uni Graz
- Aberer Elisabeth
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- Abstract:
- Lyme disease is caused by genetically divergent spirochetes, including 3 pathogenic genospecies: Borrelia burgdorferi sensu stricto, B. garinii, and B. afzelii. Serodiagnosis is complicated by this genetic diversity. A synthetic peptide (C(6)), based on the 26-mer invariable region (IR(6)) of the variable surface antigen of B. burgdorferi (VlsE), was used as ELISA antigen, to test serum samples collected from mice experimentally infected with the 3 genospecies and from European patients with Lyme disease. Regardless of the infecting strains, mice produced a strong antibody response to C(6), which indicates that IR(6) is antigenically conserved among the pathogenic genospecies. Twenty of 23 patients with culture-confirmed erythema migrans had a detectable antibody response to C(6). A sensitivity of 95.2% was achieved, with serum samples collected from patients with well-defined acrodermatitis chronica atrophicans. Fourteen of 20 patients with symptoms of late Lyme disease also had a positive anti-IR(6) ELISA. Thus, it is possible that C(6) may be used to serodiagnose Lyme disease universally.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Animals -
- Antigens, Bacterial - immunology
- Antigens, Surface - immunology
- Bacterial Proteins - immunology
- Borrelia burgdorferi Group - classification
- Enzyme-Linked Immunosorbent Assay - classification
- Female - classification
- Lipoproteins - immunology
- Lyme Disease - diagnosis
- Mice - diagnosis
- Mice, Inbred BALB C - diagnosis
- Molecular Sequence Data - diagnosis
- Sensitivity and Specificity - diagnosis
- Serologic Tests - diagnosis