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Selected Publication:
DiMagno, L; Dascal, N; Davidson, N; Lester, HA; Schreibmayer, W.
Serotonin and protein kinase C modulation of a rat brain inwardly rectifying K+ channel expressed in xenopus oocytes.
Pflugers Arch. 1996; 431(3):335-340
Doi: 10.1007/BF02207270
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Schreibmayer Wolfgang
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- Abstract:
- In Xenopus laevis oocytes injected with rat brain poly(A)+ RNA, perfusion with a high-K+ solution (96 mM KCl) generated an inward current (IHK) which was absent in water-injected oocytes. Part of IHK was blocked by low concentrations of Ba2+ (half-maximal inhibitory concentration, IC50: 4.2 +/- 0.5 microM). When serotonin (5-HT) was applied to these oocytes a transient inward oscillating Cl- current arising from activation of Ca2+ -dependent Cl- channels, ICl (Ca), was observed. When this response decayed, a 30% reduction of IHK could be detected. Electrophysiological characterization of the K+ channel down-modulated by 5-HT revealed that it is an inward rectifier. Anti-sense suppression experiments revealed that the 5-HT2C receptor mediates the down-modulatory effect of 5-HT. The nature of the modulatory pathway was investigated by application of phorbol esters and intracellular injection of protein kinase C (PKC) inhibitors, ethylenebis (oxonitrilo)tetraacetate (EGTA) and inositol 1,4,5-trisphosphate. The results demonstrate that PKC is responsible for the down-modulatory effect.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Barium - physiology
- Base Sequence - physiology
- Brain - drug effects
- Down-Regulation - drug effects
- Female - drug effects
- Molecular Sequence Data - drug effects
- Oocytes - chemistry
- Potassium Channels - biosynthesis
- Rats - biosynthesis
- Receptor, Serotonin, 5-HT2C - biosynthesis
- Receptors, Serotonin - physiology
- Serotonin - pharmacology
- Xenopus laevis - pharmacology
- Find related publications in this database (Keywords)
- Serotonin
- Inward Rectifying Potassium Channel
- Brain
- PKC
- 5-Ht2C Receptor
- Heterologous Expression
- Xenopus Laevis Oocytes
- Modulation