Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Windhager, R; Nemethova, M; Mutsaers, S; Lang, S; Kotz, R; Kitzmueller, E; Lubec, G.
Evidence for the involvement of the hydroxyl radical in the pathogenesis of excessive connective tissue proliferation in patients with tumor-endoprostheses.
Life Sci. 1998; 62(14):1261-1269
Doi: 10.1016/S0024-3205(98)00056-3
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Windhager Reinhard
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Extensive proliferation of connective tissue around Vitallium implants can be observed in young patients who had limb salvage for primary malignant bone tumors. The underlying mechanism of excess proliferation and collagen accumulation is not known. We were therefore interested to show whether the alloy of the implant induced proliferation of fibroblasts in vitro, acted by a cytotoxic mechanism or generated free radical cross linking of collagen with subsequent accumulation. In vitro tests for proliferation and cytotoxicity using the implant material which consists of a series of transition metals, ruled out a proliferation-inducing or cytotoxic effect of the implant. Determination of ortho-tyrosine (OT), a marker for hydroxyl radical attack on phenylalanine, in the proliferating tissues surrounding the implants revealed significantly higher aromatic hydroxylation in the vitallium surrounding tissue correlating with tissue collagen content (r = 0.86, p < 0.01). Based upon the findings of increased OT and the presence of higher molecular weight bands on SDS-PAGE, representing more cross linked collagen, we suggest that hydroxyl radical attack lead to free radical mediated cross linking of collagen with subsequent collagen accumulation, as collagen cross-linked to a higher degree is less susceptible to proteolytic degradation.The hydroxyl radical attack seems to having been generated by the many transitional metals of the vitallium-alloy.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Biopsy -
- Bone Neoplasms - pathology
- Case-Control Studies - pathology
- Cell Division - physiology
- Cell Survival - drug effects
- Child - drug effects
- Connective Tissue - pathology
- Female - pathology
- Fibroblasts - drug effects
- Humans - drug effects
- Hydroxyl Radical - drug effects
- Male - drug effects
- Prostheses and Implants - adverse effects
- Vitallium - adverse effects
- Find related publications in this database (Keywords)
- Vitallium (R)
- implant
- endoprosthesis
- connective tissue
- fibrosis
- free radicals
- hydroxyl radical
- orthotyrosine
- collagen
- cytoxicity
- cross linking