Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Gatschelhofer, C; Magnes, C; Pieber, TR; Buchmeiser, MR; Sinner, FM.
Evaluation of ring-opening metathesis polymerization (ROMP)-derived monolithic capillary high performance liquid chromatography columns
J CHROMATOGR A. 2005; 1090: 81-89.
Doi: 10.1016/j.chroma.2005.06.098
Web of Science
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Gatschelhofer Christina
- Sinner Frank Michael
- Co-Autor*innen der Med Uni Graz
- Pieber Thomas
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Novel monolithic capillary HPLC columns were prepared via ring opening metathesis polymerization (ROMP) within the confines of fused silica columns with 200 mu m i.d. using norborn-2-ene (NBE), 1,4,4a,5,8,8a-hexahydro- 1,4,5,8, exo, endo-dimethanonaphthalene (DMN-H6) as monomers, 2-propanol and toluene as porogens and RuCl2(PCy3)2(CHPh) as initiator. Using the monolithic capillary HPLC columns, different sets of analytes (i.e. standard systems) were used for the evaluation of the monolithic columns: (i) a protein standard consisting of six proteins in the range of 5000-66000 g/mol, (ii) an insulin-albumin standard, and (iii) a peptide standard obtained from a tryptic digest of cytochrome C. With these three different standard systems the reproducibility of synthesis in terms of separation performance proved to be 1-2% relative standard deviation in t(R). Variation of polymerization parameters had a significant influence on the monolithic morphology and therefore separation efficiency and back pressure. The maximum analytical loading capacity of ROMP-derived monolithic capillary columns for albumin was found to be 30-125ng, depending on the monomer content. Long-term stability studies showed no alteration in separation performance. (c) 2005 Elsevier B.V. All rights reserved.
- Find related publications in this database (Keywords)
- stationary phases
- LC
- monolithic columns
- capillary columns
- metathesis polymerization
- proteins
- peptides