Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Stummvoll, GH; Aringer, M; Smolen, JS; Köller, M; Kiener, HP; Steiner, CW; Bohle, B; Knobler, R; Graninger, WB.
Derangement of apoptosis-related lymphocyte homeostasis in systemic sclerosis.
Rheumatology (Oxford). 2000; 39(12):1341-1350
Doi: 10.1093/rheumatology/39.12.1341
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Graninger Winfried
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- OBJECTIVES: Both increased and decreased apoptosis may be involved in generating autoimmunity. This study addressed the question of whether apoptosis and apoptosis-regulating proteins are altered in systemic sclerosis (SSc). Patients and methods. Peripheral lymphocytes of 39 SSc patients and 47 healthy control persons were studied for apoptosis, Bcl-2 and Bax levels, expression of Fas (CD95) and activation markers (CD25, HLA-DR) as determined by fluorocytometry. Serum Fas and Fas ligand were measured by ELISA. RESULTS: SSc lymphocytes (mainly CD4(+)) expressed increased amounts of Bcl-2, while Bax was not elevated. Apoptosis rates of SSc lymphocytes were increased in unsupplemented medium, but returned to normal in the presence of autologous plasma. SSc patients had increased percentages of activated and CD95(+) lymphocytes and elevated soluble Fas and soluble FasL levels in serum. Activating anti-CD95 antibodies further increased the apoptosis rate. CONCLUSIONS: Increased in vitro apoptosis, elevated lymphocytic Bcl-2 content and the increased number of Fas-positive T cells are not specific for peripheral blood from SSc patients, but indicate deregulation of lymphocyte homeostasis in this disease.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Apoptosis -
- Enzyme-Linked Immunosorbent Assay -
- Female -
- Homeostasis -
- Humans -
- Lymphocytes - physiology
- Male -
- Middle Aged -
- Proto-Oncogene Proteins - analysis Proto-Oncogene Proteins - biosynthesis
- Proto-Oncogene Proteins c-bcl-2 - analysis Proto-Oncogene Proteins c-bcl-2 - biosynthesis
- Receptors, Interleukin-2 - analysis
- Scleroderma, Systemic - pathology Scleroderma, Systemic - physiopathology
- bcl-2-Associated X Protein -
- Find related publications in this database (Keywords)
- systemic sclerosis
- lymphocytes
- apoptosis
- cell death
- Bcl-2
- Bax
- Fas