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Selected Publication:
Bobacz, K; Gruber, R; Soleiman, A; Erlacher, L; Smolen, JS; Graninger, WB.
Expression of bone morphogenetic protein 6 in healthy and osteoarthritic human articular chondrocytes and stimulation of matrix synthesis in vitro.
Arthritis Rheum. 2003; 48(9):2501-2508
Doi: 10.1002/art.11248
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- Co-authors Med Uni Graz
- Graninger Winfried
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- Abstract:
- OBJECTIVE: To elucidate the role of bone morphogenetic protein 6 (BMP-6) in human articular cartilage, we investigated whether BMP-6 is expressed in adult human articular chondrocytes and analyzed the potential stimulatory effects of BMP-6 on these cells. In addition, we investigated whether osteoarthritic (OA) and normal cartilage chondrocytes behave differently. METHODS: Endogenous expression of the BMP-6 gene was examined by reverse transcription-polymerase chain reaction. BMP-6 protein was detected by Western immunoblotting. Chondrocytes were grown as monolayer cultures for 7 days in a chemically defined serum-free medium, in the absence or presence of recombinant BMP-6. Proteoglycan (PG) synthesis was measured by (35)S-sulfate incorporation into newly synthesized macromolecules. Cell proliferation was assessed by (3)H-thymidine incorporation. RESULTS: BMP-6 was expressed in both healthy and OA chondrocytes at the messenger RNA and protein levels. Total PG synthesis was significantly increased after BMP-6 stimulation of healthy (mean +/- SEM 191 +/- 11%; P < 0.001) and OA (150 +/- 25%; P < 0.03) chondrocyte cultures. A direct comparison between healthy and OA samples revealed no significant difference. The proliferation rates of normal and OA chondrocytes were not affected by BMP-6 treatment. CONCLUSION: BMP-6 is endogenously expressed in chondrocytes obtained from OA and normal adult human articular cartilage. Furthermore, BMP-6 has the potential to stimulate total PG synthesis in human articular chondrocytes derived from normal as well as OA joints. We conclude that the presence of BMP-6 in adult human articular cartilage indicates a functional role for this growth factor in the maintenance of joint integrity.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Aged, 80 and over -
- Bone Morphogenetic Protein 6 -
- Bone Morphogenetic Proteins - genetics Bone Morphogenetic Proteins - metabolism
- Cartilage, Articular - cytology Cartilage, Articular - physiology
- Cell Division - physiology
- Chondrocytes - cytology Chondrocytes - metabolism
- Extracellular Matrix - metabolism
- Gene Expression - physiology
- Humans -
- Middle Aged -
- Osteoarthritis - metabolism Osteoarthritis - physiopathology
- Proteoglycans - biosynthesis
- RNA, Messenger - analysis