Medizinische Universität Graz - Research portal
Selected Publication:
Hoebel, BG; Graier, WF.
11,12-Epoxyeicosatrienoic acid stimulates tyrosine kinase activity in porcine aortic endothelial cells.
Eur J Pharmacol. 1998; 346(1):115-117
Doi: 10.1016%2FS0014-2999%2898%2900118-6
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- Leading authors Med Uni Graz
- Graier Wolfgang
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- Abstract:
- Although epoxyeicosatrienoic acids, cytochrome P-450 mono-oxygenase metabolites of arachidonic acid, have been demonstrated to play a crucial role in endothelial cell Ca2+ homeostasis and endothelium-dependent vasorelaxation, the understanding of the actions of epoxyeicosatrienoic acids is limited. In this study, the effect of epoxyeicosatrienoic acids on tyrosine kinase in endothelial cell homogenate was investigated. 11,12-Epoxyeicosatrienoic acid increased tyrosine kinase activity in a concentration dependent manner (EC50 = 11.7 nM). Arachidonic acid in much higher concentrations (20 microM) mimicked the effect of the epoxyeicosatrienoic acid on tyrosine kinase. This effect of arachidonic acid was abolished in the presence of the cytochrome P-450 mono-oxygenase inhibitor thiopentone sodium, indicating that arachidonic acids needs to be converted to epoxyeicosatrienoic acids by the endothelial cytochrome P-450 mono-oxygenase to stimulate tyrosine kinase. These data describe a novel aspect of the actions of epoxyeicosatrienoic acids, and show that in addition to K+ channel activation, epoxyeicosatrienoic acids also regulate tyrosine kinase activated signaling pathways in endothelial cell activation.
- Find related publications in this database (using NLM MeSH Indexing)
- 8,11,14-Eicosatrienoic Acid - analogs and derivatives
- Animals - analogs and derivatives
- Aorta - drug effects
- Arachidonic Acid - pharmacology
- Calcium - metabolism
- Cells, Cultured - metabolism
- Endothelium, Vascular - drug effects
- Enzyme Activation - drug effects
- Homeostasis - drug effects
- Potassium Channels - metabolism
- Protein-Tyrosine Kinases - metabolism
- Swine - metabolism
- Find related publications in this database (Keywords)
- Tyrosine Kinase
- Epoxyeicosatrienoic Acid
- Endothelial