Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Herr, F; Liang, OD; Herrero, J; Lang, U; Preissner, KT; Han, VK; Zygmunt, M.
Possible angiogenic roles of insulin-like growth factor II and its receptors in uterine vascular adaptation to pregnancy.
J CLIN ENDOCRINOL METAB 2003 88: 4811-4817.
Doi: 10.1210/jc.2003-030243
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- Co-Autor*innen der Med Uni Graz
- Lang Uwe
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- Abstract:
- Adaptation of the maternal uterine vasculature is essential for normal fetal and placental development in which angiogenesis is considered one of the most critical adaptive changes during pregnancy. Highly expressed in cytotrophoblasts and maternal endothelial cells during pregnancy, IGF-II promotes cell migration and regulates fetal and placental growth. We hypothesized that IGF-II regulates uterine angiogenesis during pregnancy. Both uterine vasculature and isolated uterine microvascular endothelial cells expressed high levels of IGF-II and IGF-II/mannose-6 phosphate receptor mRNA as shown by in situ hybridization. Physiological concentrations of IGF-II significantly increased vessel formation, as shown by a three-dimensional angiogenesis assay in vitro or a chicken chorionallantoic membrane assay in vivo. The angiogenic response of IGF-II could be reversed by the addition of beta-galactosidase or rabbit-antihuman IGF-II/M6P receptor antiserum, whereas blocking antibodies against IGF-I receptor or insulin receptor influenced IGF-II-induced sprout formation. IGF-II promoted migration of endothelial cells (10-250 ng/ml) tested in a modified Boyden chamber, but no stimulating effect on proliferation was observed. The application of several intracellular signal transduction molecules and their inhibitors indicated that protein kinase C and G(i) protein might play a role in the IGF-II-induced angiogenesis. Our results suggest an important angiogenic role of IGF-II in the vascular adaptation to pregnancy.
- Find related publications in this database (using NLM MeSH Indexing)
- Adaptation, Physiological - physiology
- Animals - physiology
- Cell Division - drug effects
- Cell Line - drug effects
- Cell Movement - drug effects
- Chick Embryo - drug effects
- Cross-Linking Reagents - metabolism
- Endothelium, Vascular - cytology
- Female - cytology
- Gene Expression - physiology
- Humans - physiology
- Insulin-Like Growth Factor II - genetics
- Iodine Radioisotopes - diagnostic use
- Neovascularization, Physiologic - drug effects
- Pregnancy - drug effects
- RNA, Messenger - analysis
- Receptor, IGF Type 2 - genetics
- Umbilical Veins - cytology
- Uterus - blood supply